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> CD146 (MCAM) Rat Monoclonal Antibody [Clone ID: OJ79c]

CD146 (MCAM) Rat Monoclonal Antibody [Clone ID: OJ79c]

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Référence SM1860PTS

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MCAM (Melanoma Cell Adhesion Molecule) / MUC18 / CD146 Mouse Monoclonal Antibody [Clone ID: OJ79c]

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SKU
SM1860PTS
MCAM (Melanoma Cell Adhesion Molecule) / MUC18 / CD146 mouse monoclonal antibody, clone OJ79c, Purified
Size:
20 ug
Conjugation:
Unconjugated
 

View other "MCAM or NP_006491.2" antibodies

Specifications
Product Data
Clone Name OJ79c
Application FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use BSA free antibody for coating.
Flow Cytometry: 0.5-1 µg/106 cells.
Immunofluorescence: 0.5-1 µg/ml.
Western Blot: 0.5-1 µg/ml.
Immunoprecipitation: 1-2 μg/500 µg protein lysate.
Immunohistochemistry on Frozen Sections: 0.5-1 µg/ml for 30 minutes at RT.
Positive Control: A-375, HUV-EC or HeLa Cells, Tonsil or Melanoma.
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant full-length human MUC18 protein
Specificity Cellular Localization: Membrane
Buffer 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size 130kDa
Gene Name melanoma cell adhesion molecule
Database Link
Background The human Mel-CAM gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. Mel-CAM is found on 80% of advanced primary human melanomas and correlates well with development of metastatic disease.
Synonyms Cell Surface Glycoprotein MUC18, Cell Surface Glycoprotein P1H12, Gicerin, Melanoma Adhesion Molecule (MCAM), Melanoma Associated Glycoprotein MUC18, Melanoma Cell Adhesion Molecule, Melanoma-associated Antigen A32, Mel-CAM, S-endo 1 Endothelial-associated Antigen, Sendo1
Reference Data
Product Manuals
FAQs
  • Antibody and Western Standard
SDS

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