Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (PE)

CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (PE)

Imprimer Imprimer
Identifiez-vous

Référence C2253-02B1-PE-100ul

Conditionnement : 100ul

Marque : US Biological

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

C2253-02B1-PECD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (PE)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcebovine
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC IHC IP
CrossreactivityBo Gt
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:20; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: CC42|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD2. Species crossreactivity: goat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD2. Species crossreactivity: goat.
References
1. Davis, W.C. and Splitter, G.S. (1991) Individual antigens of cattle. Bovine CD2 (BoCD2). Vet. Immunol. Immunopathol. 27: 43-50, 2. Sopp, P et al. (1991) Investigating monoclonal antibodies to bovine "null" cell antigens using two-colour immunofluorescence. Vet. Immunol. Immunopathol. 27: 163-168, 3. Eskra, L et al. (1991) Effect of monoclonal antibodies on in vitro function of T-cell subsets. Vet. Immunol. Immunopathol. 27: 215-229, 4. Gutierrez, M. et al. (1999) The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet. Immunol. Immunopathol. 71: 321-334.