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> CD25 (IL2RA, Interleukin-2 Receptor Subunit alpha, TAC Antigen, p55) (APC)

CD25 (IL2RA, Interleukin-2 Receptor Subunit alpha, TAC Antigen, p55) (APC)

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Référence 367060-100tests

Conditionnement : 100tests

Marque : US Biological

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367060CD25 (IL2RA, Interleukin-2 Receptor Subunit alpha, TAC Antigen, p55) (APC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcehuman
Swiss ProtP01589
IsotypeIgG2a,k
GradeHighly Purified
ApplicationsFC
CrossreactivityHu
Accession #NM_000417
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

The antibody specifically binds to the 55kD type I transmembrane glycoprotein known as the low-affinity interleukin-2 receptor alpha chain subunit (IL2Ra, CD25). CD25 is expressed on regulatory T cells and on activated lymphocytes (T and B) and monocytes. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 7G7B6|Host: mouse|Source: human|Concentration: ~0.05mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC). |Purity: Highly Purified|Immunogen: Influenza virus stimulated human peripheral blood mononuclear cells|Specificity: Recognizes human CD25. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Influenza virus stimulated human peripheral blood mononuclear cells
Form
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Purity
Highly Purified
Specificity
Recognizes human CD25.
References
1. Boutin B, et al. 1989. Neuropediatrics. 20:202. 2. Lundin K, et al. 2001. Anal Biochem. 299:92. 3. Raivio E, et al. 1997. APMIS. 105:108. 4. Yamamura T, et al. 2002. Arch Dermatol Res. 294:349.