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> CD28 IgG2a Fc, Fusion Protein

CD28 IgG2a Fc, Fusion Protein

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Référence C2380-03E-25ug

Conditionnement : 25ug

Marque : US Biological

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C2380-03E CD28 IgG2a Fc, Fusion Protein

Grade
Affinity Purified
Applications
E
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60 kd and 80 kd, respectively.

Molecular Structure: A soluble fusion protein consisting of the extracellular (134 aa) domain of  human CD28 fused to murine IgG2a Fc (232 aa), with a predicted monomeric molecular weight of 41.8kD.

Human CD28-muIg Fusion Protein

Transfectant Cell Line: CHO  

Functional Application: CD28-muIg fusion protein blocks binding of anti-human CD28 to human tumor cells.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Source: CHO cells|Purity: Purified by Protein A affinity chromatography.|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.
Purity
Purified by Protein A affinity chromatography.
References
(1.) M.K. Jenkins & J.G. Johnson, (1993) Curr Opin Immunol 5: 361-367. |(2.) 61st Forum in Immunology, (1995) Res Immunol 146: 127-205. |(3.) I. Kariv, A. Truneh & R.W. Sweet, (1996) J Immunol 157: 29-38. |(4) R.J. Peach, Linsley P.S., et al. J Biol Chem (1995) 270(36): 21181-21187.