CD28 (PE)

Référence 214643-PE-100ul

Conditionnement : 100ul

Marque : US Biological

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214643-PECD28 (PE)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcechicken
IsotypeIgG1
GradeHighly Purified
ApplicationsFC IP
CrossreactivityCh
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

The chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive (Koskela et al. 1998). The co-stimulatory role of CD28 is important to T cell function in chickens (Arstila et al. 1994).

Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: AV7|Host: mouse|Source: chicken|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Ion Exchange chromatography from tissue culture supernatant.|Immunogen: Chicken Leucocytes|Specificity: Recognizes chicken CD28. Species Crossreactivity: Turkey||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Chicken Leucocytes
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Ion Exchange chromatography from tissue culture supernatant.
Specificity
Recognizes chicken CD28. Species Crossreactivity: Turkey
References
1. Powell F et al (2009) Development of reagents to study the turkey's immune response: Identification and molecular cloning of turkey CD4, CD8a and CD28 Dev. & Comp. Immunology 33:540-546 2. Young, J.R. et al. (1994) Monomeric homologue of mammalian CD28 is expressed on chicken T cells J. Immunol. 152: 3848-3851.