CD3 (FITC)

CD3 is closely associated on the lymphocyte cell surface with the T cell antigen receptor TcR. It is believed that the CD3 complex transmits activational signals to the T cell’s interior upon antigen recognition by TcR. CD45 (LCA), a protein tyrosine phosphatase, is a potent regulator of signal transduction by the CD3 complex. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukemia cells, Sézary leukemias and approximately 70% of acute lymphoblastic leukemias of T cell origin.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:5-1:10. Membrane permeabilization is required.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.