CD35 (CR1, C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen) (PE)

Recognizes a protein of 210-220kD, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

Applications:
Suitable for use in FLISA, Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-1ug/10e6 cells
Western Blot: 0.5-1ug/ml
Immunoprecipitation: 1-2ug/500ug protein lysate
Immunofluorescence: 1-2ug/ml
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Follicular dendritic cells (FDC) in tonsil

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.