CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (PerCP/Cy5.5)

CD4 is a 55kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is a specific marker for T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. 367091 binds to the D3 domain of CD4 and does not inhibit HIV binding.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.