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 FCM staining of human PBMC's using CD5 Monoclonal Antibody (CRIS-1).
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Product Description
Recognizes a 67 kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF ® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.
Catalog number key for antibody number 0176, Anti-CD5 (Cris-1)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified,
BSA-free | N/A | N/A | N/A | |
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
Technology
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Product Attributes
Antibody reactivity (target)
CD5
Synonyms
CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5
Immunogen
Stimulated human leukocytes
Antibody target cellular localization
Plasma membrane
Verified antibody applications
Flow (verified)
Species reactivity
Human, Rhesus monkey
Expected antibody applications
ELISA (published for clone), Functional studies (published for clone), IP (published for clone)
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Immunofluorescence 0.5-1 ug/mL Immunoprecipitation 0.5-1 ug/500 ug protein lysate, Optimal dilution for a specific application should be determined by user
Positive control
293T, Ramos, or MOLT-4 Cells. Tonsil.
Shipping condition
Room temperature
Storage Conditions
Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas
Immunology
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression
Lymphocytes, T-cells
Tumor expression
Leukemia/lymphoma
Documents, Protocols, SDS and COA
Datasheet
Datasheet Download
COARequest the Certificate of Analysis
References
Note: References for this clone sold by other suppliers may be listed for expected applications. - Immunology (1990) 70, 434-439. (Flow)
- J Immunol (1992) 148(5):1287-93. (receptor activation)
- J Immunol (1993) 151 (9): 4423-4430. (IP)
- Nature (1991) 351: 662-665. (ELISA)
Citations
Note: Note: Listed references are for this antibody clone sold by Biotium and other suppliers.
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