CD61 (Integrin beta-3, Platelet Membrane Glycoprotein IIIa, GPIIIa, ITGB3, GP3A) (APC)

CD61, known as integrin b3 or glycoprotein IIIa (gpIIIa), is a 90 kD type I integral transmembrane glycoprotein. It is a member of integrin family that associates with platelet gpIIb (CD41) forming CD41/CD61 complex and associates with integrin aV (CD51) forming aV/b3 (CD51/CD61) integrin. CD41/CD61 is expressed on platelets and megakaryocytes, and plays a role in platelet activation and aggregation through interaction with fibrinogen, fibronectin, vWF and other RGD-containing adhesion molecules. CD51/CD61 is expressed on platelets, osteoclasts, fibroblast, macrophages and some tumor cells, involved in tumor metastasis and in adenovirus infection through binding to RGD motif ECM proteins.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 5ul/10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.