Cd68 Antibody - FITC Conjugated
Référence OASA04076
Conditionnement : 0.1mg
Marque : Aviva Systems Biology
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Cd68 Antibody - FITC Conjugated (OASA04076)
| Datasheets/Manuals | Printable datasheet for Cd68 Antibody - FITC Conjugated (OASA04076) |
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| Predicted Species Reactivity | Rat |
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| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | ED1 |
| Isotype | IgG1 |
| Host | Mouse |
| Conjugation | FITC |
| Application | FC |
| Additional Information | Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line. |
| :: | Max Ex/Em: 490nm/525nm |
| Reconstitution and Storage | -20°C |
| Immunogen | Rat spleen cells. |
| Purification | Protein A Purified |
| Predicted Homology Based on Immunogen Sequence | Rat |
| Concentration | 0.1 mg/ml |
| Specificity | CD68. Reacts with bovine. Does not react with horse. |
| Application Info | Flow Cytometry: Neat. Membrane permeabilization is required for this application. |
| Application Data | Application #1: Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68  Application #2: Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm) (BUF09)  Application #3: Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:FITC following permeabilisation with Leucoperm (BUF09)  Application #4: Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm (BUF09)  Application #5: Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 488 . following permeabilisation with Leucoperm (BUF09)  |
| Protocol Information | Citation: 1: Huang W, Metlakunta A, Dedousis N, Zhang P, Sipula I, Dube JJ, Scott DK, O'Doherty RM. Depletion of liver Kupffer cells prevents the development of diet-induced hepatic steatosis and insulin resistance. Diabetes. 2010 Feb;59(2):347-57. Epub 2009 Nov 23. PubMed PMID: 19934001; PubMed Central PMCID: PMC2809951. Species: Rat Experiment Name: Immunohistochemistry and immunofluorescence. Experiment Background: In the current study, depletion of Kupffer cells was evaluated by quantification of CD68 (a macrophage marker)-positive cells by immunohistochemistry Experimental Steps: 1. For immunohistochemistry, tissues (liver and epididymal fat of Rat) were fixed overnight in a 4% paraformaldehyde solution, and subsequently embedded in paraffin.2. Sections were obtained and stained using standard techniques with CD68.CD68-positive cells were detected using α-CD68 (AbD ) and were quantified on 8–9 fields of ×40 sections using an Olympus (New York, NY) light microscope.3. For immunofluorescence, CD68 and TNFα were detected in liver sections after antigen retrieval by heating sections in 1.6 mmol/l EDTA in a high-pressure cooker for 7 min.4. Subsequently, sections were immunostained with α-CD68 (1:200) and α-TNFα (1:100; MBL, Woburn, MA) followed by incubation with Alexa 488–conjugated or cyanin 3–conjugated secondary antibodies, respectively. Images were visualized using an Olympus Provis fluorescence microscope and modified with Magnafire software (Olympus, Melville, NY). Other Reagents Used: Gadolinium chloride, NaH2PO4, FBS, glucose. Number Of Protocols: 2 |
| Reference | 1. Damoiseaux, J.G. et al. (1994) Rat macrophage lysosomal membrane antigen recognised by monoclonal antibody ED1. Immunol. 83: 140-147. 2. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognised by monoclonal antibodies ED1, ED2 and ED3. Immunol 54: 589-599. 3. Bauer, J. et al. (1994) Phagocytic activity of macrophages and microglial cells during the course of Acute and Chronic Relapsing Experimental Autoimmune Encephalomyelitis. J. Neurosci. Res. 38: 365-375. 4. Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spinal cord injury in rats. J, Neuro-chem. 88:1335-1344. 5. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and |
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| Gene Symbol | Cd68 |
| Alias Symbols | MGC114377, Cd68 |
| NCBI Gene Id | 287435 |
| Protein Name | Cd68 molecule EMBL AAH98931.1 |
| Description of Target | MOUSE ANTI RAT CD68:FITC |
| Uniprot ID | Q4FZY1 |
| Protein Accession # | NP_001026808.1 |
| Protein Size (# AA) | 330 |
