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> CD80 (B7-1) (PE)

CD80 (B7-1) (PE)

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Référence 214743-PE-100ul

Conditionnement : 100ul

Marque : US Biological

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214743-PECD80 (B7-1) (PE)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcebovine
Swiss ProtO46405
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC
CrossreactivityBo Sh
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

CD80 cell surface antigen is expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells in the primary immune response.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: IL-A159|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD80. Species Crossreactivity: sheep ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine CD80. Species Crossreactivity: sheep
References
1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells. Scand J Immunol. 61: 242-50 2. Rhodes, S.G. et al. (2003) 1,25-Dihydroxyvitamin D3 and development of tuberculosis in cattle. Clin. Diag. Lab. Immunol. 10: 1129-35. 3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells. J. Gen. Virol. 84: 1771-1780. 4. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP– subset in lymph dendritic cells from the upper aero-digestive tract. J. Leukoc. Biol. 76:553-561. 5. Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node. J Leukoc Biol. 79: 268-76. 6. Hart, J. et al. (2011) Theileria annulata-transformed cell lines are efficient antigen-presenting cells for in vitro analysis of CD8 T cell responses to bovine herpesvirus-1. Vet Res. 42: 119. 7. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro. Vet Res. 44: 59.