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> CD86 (B7-2) (Biotin)

CD86 (B7-2) (Biotin)

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Référence 214748-Biotin-100ul

Conditionnement : 100ul

Marque : US Biological

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214748-BiotinCD86 (B7-2) (Biotin)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcebovine
IsotypeIgG1
GradeAffinity Purified
ApplicationsFC
CrossreactivityBo Sh
Shipping TempBlue Ice
Storage Temp-20°C

CD86 cell surface antigen, which is expressed by dendritic cells, activated macrophages and activated B cells. CD86 plays an important role in co-stimulation of T cells in the primary immune response.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: IL-A190|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography.|Specificity: Recognizes bovine CD86. Species Crossreactivity: sheep ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes bovine CD86. Species Crossreactivity: sheep
References
1. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells. Scand J Immunol. 61: 242-50 2. Rhodes, S.G. et al. (2003) 1,25-Dihydroxyvitamin D3 and development of tuberculosis in cattle. Clin. Diag. Lab. Immunol. 10: 1129-35. 3. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells. J. Gen. Virol. 84: 1771-1780. 4. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP– subset in lymph dendritic cells from the upper aero-digestive tract. J. Leukoc. Biol. 76:553-561. 5. Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node. J Leukoc Biol. 79: 268-76. 6. Norimatsu, M. et al. (2003) Differential response of bovine monocyte-derived macrophages and dendritic cells to infection with Salmonella typhimurium in a low-dose model in vitro. Immunology. 108: 55-61. 7. Hemati, B. et al. (2009) Bluetongue virus targets conventional dendritic cells in skin lymph. J Virol. 83: 8789-99. 8. Pascale, F. et al. (2008) Plasmacytoid dendritic cells migrate in afferent skin lymph. J Immunol. 180: 5963-72. 9. Ruscanu, S. et al. (2012) The double-stranded RNA bluetongue virus induces type I interferon in plasmacytoid dendritic cells via a MYD88-dependent TLR7/8-independent signaling pathway. J Virol. 2012 May;86: 5817-28.