Conditionnement : 25mg
Téléphone : +1 850 650 7790
Synonyms:
| Description | CelGreen nucleic acid gel stain *10,000× concentrate in water* is a fluorescent dye with a unique involvement that binds to all dsDNA double helix sulcus regions.In the free state, the side strand and CelGreen emit weak fluorescence, but the fluorescence is greatly enhanced once they are combined with the double-stranded DNA. Therefore, the fluorescence signal strength of CelGreen is related to the amount of double-stranded DNA, and the amount of double-stranded DNA in the PCR system can be detected according to the fluorescence signal. |
| In vitro | GelGreen for Electrophoresis In Gel Staining (Pre-staining, same method as EB) Prepare a agarose solution and heat till it dissolves. GelGreen Nucleic Acid Staining solution is diluted from the GelGreen Nucleic Acid Stain 1:10,000 prior to pouring the gel. Since good thermal stability, GelGreen can be added directly to hot agarose solution. Shake to ensure being fully mixed flowing by cast the gel, Run gel and view results by UV projector. |
| Relative Density. | no data available |
| Storage | Keep away from direct sunlight Store at -20°C Shipping with blue ice/Shipping at ambient temperature. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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