Cell Counting Kit-8 (CCK-8)
Référence MBS9719068-5x10K
Conditionnement : 5x10KTests
Marque : MyBiosource
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Cell Counting 8 (CCK-8) Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9719068Principle of the Assay: Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8. [2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a… More details
Product Overview
Online Datasheet in PDFProduct Name
Cell Counting 8 (CCK-8), Assay Kit
Full Product Name
Cell Counting Kit-8 (CCK-8)
Product Gene Name
CCK-8 assay kit
Product Synonym Names
CCK-8; WST-8; CCK8; WST8
Form/Format
Liquid solution
Preparation and Storage
CCK-8 is stable for one year at 0-5 degree C with protection from light. Store it at -20 degree C for longer storage. Repeated thawing and freezing causes an increase in the background, which interferes with the assay. Please store the kit at 0-5 degree C for frequent use.
Shipping: Gel pack with blue ice.
Shipping: Gel pack with blue ice.
Kit Components
CCK-8 solution
Features & Benefits
One-bottle, ready-to-use solution.
The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe.
The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
The same cells can be used for other cell assays because of the low toxicity of CCK-8.
The product (formazan) produced by WST-8 is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe.
The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
The same cells can be used for other cell assays because of the low toxicity of CCK-8.
Usage Notes
Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.
Be careful not to introduce bubbles to the wells, since they interfere with the OD reading.
The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.
If the color or PH of culture media is changed due to long-time culture, please change the culture media when adding CCK-8.
The same cells can be used for other cell assays because of the low toxicity of CCK-8.
Be careful not to introduce bubbles to the wells, since they interfere with the OD reading.
The incubation time varies by the type and number of cells in a well. Generally, leukocytes give weak coloration, thus a long incubation time (up to 4 hours) or a large number of cells (~105 cells/well) may be necessary.
If the color or PH of culture media is changed due to long-time culture, please change the culture media when adding CCK-8.
The same cells can be used for other cell assays because of the low toxicity of CCK-8.
Product Categories/Family for CCK-8 assay kit
Cell Status Detection
Other Notes
Small volumes of CCK-8 assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for CCK-8 purchase
MBS9719068 is an assay kit targeting CCK-8. Also known as Cell Counting Kit-8 (CCK-8).