CheKine™ Micro Lactate Assay Kit
Référence KTB1100-96T×5
Conditionnement : 96Tx5/480S
Marque : Abbkine
Specification
| Product name | CheKine™ Micro Lactate Assay Kit |
| Applications notes | CheKine™ Micro Lactate Assay Kit provides a convenient means for detecting L (+) -Lactate in biological samples such as in serum or plasma, cells, culture and fermentation media. In this kit lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a tetrazolium salt WST-8 dye to form a colorimetric (450 nm) product, proportional to the lactate present. |
Product Properties
| Kit components | • Lactate Assay Buffer • Lactate Dehydrogenase • Lactate Dehydrogenase Cofactor • WST-8 • Enhancer • L (+) -Lactate Standard (100 mM) |
| Features & Benefits | • Determination of L (+) -Lactate in serum, plasma, cell/tissue samples, plant tissues,urine and other biological fluids. • Detect biological samples at a level of 0.03 mM-2 mM. • Pre-treatment or purification of samples are not required. • Using WST-8 as substrate, more sensitive than MTT. |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month. • It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range. • Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Lactate is an important intermediate product in the metabolic process of organisms, which is closely related to glucose metabolism, lipid metabolism, protein metabolism and intracellular energy metabolism. The content of lactate is an important indicator to evaluate glycogen metabolism and aerobic metabolism. |
Image & description
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α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure(KTB1100-25.9)
Author:N Zhang, Y Zhang, J Xu, P Wang, B Wu, S Lu, X Lu, ... Publication name:Scientific Reports IF:25.9
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ZNF207-Driven PRDX1 Lactylation and NRF2 Activation in Regorafenib Resistance and Ferroptosis Evasion.(KTB1100-21.7)
Author:Yang, Tianfeng, et al. Publication name:Drug Resistance Updates IF:21.7
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Divalent metal ions enhance bone regeneration through modulation of nervous systems and metabolic pathways.(KTB1100-20.3)
Author:Luo, Ying, et al. Publication name: Bioactive Materials IF:20.3
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Targeting macrophage polarization by inhibiting Pim2 alleviates inflammatory arthritis via metabolic reprogramming.(KTB1100-19.8)
Author:Xu, Xiaojun, et al. Publication name:Cellular & Molecular Immunology IF:19.8
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The S-depalmitoylase ABHD10 is essential for sperm mitochondrial sheath formation and male fertility(KTB1100-15.7)
Author:S Zhou, H Zhou, H Xu, M Xiong, S Gan, D Liu, ... Publication name:Acta Pharmacol Sin IF:15.7
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Dual activation of GCGR/GLP1R signaling ameliorates intestinal fibrosis via metabolic regulation of histone H3K9 lactylation in epithelial cells(KTB1100-14.7)
Author:Han Liu Publication name:Aquaculture Reports IF:14.7
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ARL5B Drives Esophageal Squamous Cell Carcinoma Progression via ROCK1–SREBP1‐Mediated Lipid Metabolic Reprogramming(KTB1100-14.1)
Author:X Ma, Y Sun, H Mao, C Huang, Z Li, T Wang, ... Publication name:cancer science IF:14.1
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High Sugar Induced RCC2 Lactylation Drives Breast Cancer Tumorigenicity Through Upregulating MAD2L1.(KTB1100-14.1)
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METTL14‐Mediated M6A Modification of LINC01094 Induces Glucose Metabolic Reprogramming in Breast Cancer by Recruiting the PKM2/JMJD5 Complex.(KTB1100-14.1)
Author:Wang, Mengqi, et al. Publication name:Advanced Science IF:14.1
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UGDH lactylation aggravates osteoarthritis by suppressing glycosaminoglycan synthesis and orchestrating nucleocytoplasmic transport to activate MAPK signaling.(KTB1100-14.1)
Author:Lan, Weiren, et al. Publication name: Advanced Science IF:14.1
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TNF‐α‐Induced KAT2A Impedes BMMSC Quiescence by Mediating Succinylation of the Mitophagy‐Related Protein VCP.(KTB1100-14)
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DLGAP5 enhances bladder cancer chemoresistance by regulating glycolysis through MYC stabilization.(KTB1100-13.3)
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Kindlin-2 enhances c-Myc translation through association with DDX3X to promote pancreatic ductal adenocarcinoma progression(KTB1100-13.3)
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Enhancing photocatalytic hydrogen production from engineered Escherichia coli-biohybrid system via intracellular electron redirection.(KTB1100-13)
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Lactate exacerbates lung damage induced by nanomicroplastic through the gut microbiota–HIF1a/PTBP1 pathway(KTB1100-12.8)
Author:L Xuan, Z Xu, J Luo, Y Wang, Y Yan, C Qu, ... Publication name:Front Immunol IF:12.8
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CDCA3-MYC positive feedback loop promotes bladder cancer progression via ENO1-mediated glycolysis.(KTB1100-12.8)
Author:Shen, Dexin, et al. Publication name:Journal of Experimental & Clinical Cancer Research IF:12.8
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Human papillomavirus-16 E6 activates the pentose phosphate pathway to promote cervical cancer cell proliferation by inhibiting G6PD lactylation(KTB1100-11.9)
Author:孟庆飞 Publication name:Neuroscience IF:11.9
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Mitophagy Reprograms Lactate Metabolism to Suppress THBS1 via H3K18la Reduction, Alleviating Intervertebral Disc Degeneration(KTB1100-10.7)
Author:K Wang, X Wu, H Li, X Xu, F Cheng, J Chen, H Zhou, ... Publication name:International Journal of Molecular Medicine IF:10.7
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“All-in-one” metal polyphenol network nanocapsules integrated microneedle patches for lipophagy fueled ferroptosis-mediated multimodal therapy(KTB1100-10.5)
Author:王文浩 Publication name:Respiratory Research IF:10.5
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RHBDF1 deficiency suppresses melanoma glycolysis and enhances efficacy of immunotherapy by facilitating glucose-6-phosphate isomerase degradation …(KTB1100-10.5)
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Gap junctions mediate glucose transfer to promote colon cancer growth in three-dimensional spheroid culture(KTB1100-10.1)
Author:K Gong, Q Hong, H Wu, F Wang, L Zhong, L Shen, ... Publication name:Microbiology Spectrum IF:10.1
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dsHMGB1, released from IL-17A-induced pyroptotic prostate epithelial cells, drives M1 polarization by promoting Pfkp-mediated glycolysis via Jak2/Stat1 …(KTB1100-10)
Author:W Ma, Y Zhang, W Xu, Y Hu, W Wu, ... Publication name: IF:10
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IGF1R Enhances Calcium Oxalate Monohydrate-Induced Epithelial-Mesenchymal Transition by Reprogramming Metabolism via the JAK2/STAT3 Signaling.(KTB1100-10)
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Physical Encapsulation and Chemotherapy: A Synergistic Hydrogel Strategy for Tumor Suppression(KTB1100-10)
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Targeting the HSP60/p53 Axis with Extracellular Vesicle-Delivered siRNA Reprograms Glycolysis in Prostate Cancer(KTB1100-10)
Author:Meng-Yao Xu (徐梦瑶) Publication name:Biochim Biophys Acta IF:10
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MRPL12 K163 acetylation inhibits ccRCC via driving mitochondrial metabolic reprogramming(KTB1100-9.6)
Author:X Ji, F Xue, Y Wang, D Gao, J Sun, T Dong, Q Mu, ... Publication name:Journal of Ethnopharmacology IF:9.6
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Pump‐Free Unidirectional Vertical Centrifugal Microfluidic Platform for Dynamic 3D Tumor Cluster Culture and Functional Drug Screening(KTB1100-9.6)
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KAT7 enhances the proliferation and metastasis of head and neck squamous carcinoma by promoting the acetylation level of LDHA.(KTB1100-9)
Author:Lu, Ying, et al. Publication name:Cancer Letters IF:9
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CXCR4 regulates macrophage M1 polarization by altering glycolysis to promote prostate fibrosis.(KTB1100-8)
Author:Zhang, Yi, et al. Publication name:Cell Communication and Signaling IF:8
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TAB182 regulates glycolytic metabolism by controlling LDHA transcription to impact tumor radiosensitivity.(KTB1100-8)
Author:Chen, Shi, et al. Publication name:Cell Death & Disease IF:8
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Targeting the PDK/PDH axis to reverse metabolic abnormalities by structure-based virtual screening with in vitro and in vivo experiments.(KTB1100-8)
Author:Yue, Jianda, et al. Publication name:International Journal of Biological Macromolecules IF:8
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Glucose-induced LINC01419 reprograms the glycolytic pathway by recruiting YBX1 to enhance PDK1 mRNA stability in hepatocellular carcinoma(KTB1100-7.9)
Author:刘艳芳 Publication name:Asian-Australasian Journal of Animal Sciences IF:7.9
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Lactate Facilitates Pancreatic Repair Following Acute Pancreatitis by Promoting Reparative Macrophage Polarization.(KTB1100-7.4)
Author:Jiang, Jing, et al. Publication name:Cellular and Molecular Gastroenterology and Hepatology IF:7.4
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Leveraging AI technology for distinguishing Eucommiae Cortex processing levels and evaluating anti-fatigue potential.(KTB1100-6.3)
Author:Pan, Yijing, et al. Publication name:Computers in Biology and Medicine IF:6.3
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Comprehensive bioinformatics analysis of the solute carrier family and preliminary exploration of SLC25A29 in lung adenocarcinoma(KTB1100-6)
Author:P Zheng, Z Mao, M Luo, L Zhou, L Wang, H Liu, ... Publication name: IF:6
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The potential role of CMC1 as an immunometabolic checkpoint in T cell immunity.(KTB1100-6)
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Glycolysis regulates palatal mesenchyme proliferation through Pten-Glut1 axis via Pten classical and non-classical pathways.(KTB1100-5.9)
Author:Wang, Yijia, et al. Publication name: Cell Biology and Toxicology IF:5.9
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Neutrophil-derived heparin-binding protein increases endothelial permeability in acute lung injury by promoting TRIM21 and the ubiquitination of P65.(KTB1100-5.9)
Author:Zhang, Jian, et al. Publication name: Cell biology and toxicology IF:5.9
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Baicalin promotes anti-tumor immunity in hepatocellular carcinoma through HIF-1α/Lactate/CXCL9 axis.(KTB1100-5.6)
Author:Yang, Xuemei, et al. Publication name:Biochemical Pharmacology IF:5.6
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Gingerenone A inhibits LDHA-mediated glycolysis and restores sunitinib sensitivity in renal cell carcinoma(KTB1100-5.6)
Author:Z Chen, Y Zhang, L Ma, Y Song, W Li, Z Sun, ... Publication name:Research Square IF:5.6
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Natural product fargesin interferes with H3 histone lactylation via targeting PKM2 to inhibit non‐small cell lung cancer tumorigenesis.(KTB1100-5)
Author:Guo, Zizhang, et al. Publication name:BioFactors IF:5
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Cucurbitacin B from Cucurbitaceae Plants: Treating Pancreatic Cancer via Inducing Mitophagy, Inhibiting Glycolysis, and Enhancing Immune Function(KTB1100-5)
Author:D Yin, H Chen, X Jing, S Lin, Y Sun, R Chang, Y Feng, ... Publication name:Exp Neurol IF:5
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Hypoxia promotes histone H3K9 lactylation to enhance LAMC2 transcription in esophageal squamous cell carcinoma.(KTB1100-5)
Author:Zang, Yong, et al. Publication name:Iscience IF:5
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Oncogenic HPV-induced high expression of ESM1 predicts poor prognosis and regulates aerobic glycolysis in cervical cancer."(KTB1100-5)
Author:Yuan, Lin, et al. Publication name:Iscience IF:5
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Targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma.(KTB1100-5)
Author:Wang, Yinghao, et al. Publication name:Iscience IF:5
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Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation.(KTB1100-4.9)
Author:Sun, Zhe, et al. Publication name: PLoS pathogens IF:4.9
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Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the …(KTB1100-4.9)
Author:Z Li, Y Jiang, J Liu, H Fu, Q Yang, ... Publication name:Antioxidants IF:4.9
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Jaceosidin Attenuates Sepsis-Induced Myocardial Dysfunction by Promoting SIRT2-Mediated Inhibition of Histone H3K18 Lactylation(KTB1100-4.8)
Author:H Yu, M Liu, S Hou, J Wu, Q Du, F Feng, S Wang, ... Publication name:Cell death discovery IF:4.8
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Gastrodin regulates H3K14la through the CDT2-KAT2A axis to treat Sepsis-induced myocardial dysfunction.(KTB1100-4.7)
Author:Yu, Huiming, et al. Publication name:International Immunopharmacology IF:4.7
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Oral delivery of dextran-modified albumin nanoparticles loaded with shikonin for targeted therapy of colorectal cancer(KTB1100-4.5)
Author:Z Ren, J Zhao, S Li, Y Hong Publication name:BioRxiv IF:4.5
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DANCR promotes septic cardiomyopathy by enhancing macrophage glycolytic reprogramming via the IGF2BP2/HK2 axis(KTB1100-4.3)
Author:C Ma, S Liu, Y Zhao, H Liu, X Zhang Publication name:Journal of Translational Medicine IF:4.3
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Remote ischemic postconditioning-mediated neuroprotection against stroke by promoting ketone body-induced ferroptosis inhibition.(KTB1100-4)
Author:Huang, Lin-yan, et al. Publication name:ACS chemical neuroscience IF:4
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DRAM1 enhances the proliferation and metastasis of gastric cancer through the PI3K/AKT/mTOR signaling pathway and energy metabolism.(KTB1100-3.9)
Author:Wu, Xinrong, et al. Publication name:Scientific Reports IF:3.9
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IGF2BP1 stabilizes Akt2 mRNA to promote glucose metabolism and maintain spermatogonial proliferation.(KTB1100-3.7)
Author:Liu, Yanan, et al. Publication name: Reproduction IF:3.7
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Vitamin C inhibits the growth of colorectal cancer cell HCT116 and reverses the glucose-induced oncogenic effect by downregulating the Warburg effect(KTB1100-3.5)
Author:C Yu, S Min, F Lv, L Ren, Y Yang, L Chen Publication name:FASEB JOURNAL, 17 Jan 2019 IF:3.5
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A Novel Prognostic Signature Integrating Immune and Glycolytic Pathways for Enhanced Prognosis and Immunotherapy Prediction in Hepatocellular Carcinoma.(KTB1100-3.4)
Author:Zhang, Zeyu, et al. Publication name: Journal of Hepatocellular Carcinoma IF:3.4
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Vitamin D-VDR (vitamin D receptor) alleviates glucose metabolism reprogramming in lipopolysaccharide-induced acute kidney injury(KTB1100-3.4)
Author:Q Dai, H Zhang, S Tang, X Wu, J Wang, B Yi, ... Publication name:Biology IF:3.4
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Exosomes from cisplatin-induced dormant cancer cells facilitate the formation of premetastatic niche in bone marrow through activating glycolysis of BMSCs(KTB1100-3.3)
Author:J Xu, X Feng, N Yin, L Wang, Y Xie, Y Gao, ... Publication name:ACS Nano IF:3.3
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SUMOylated GLUT1 inhibited the glycometabolism disorder in chondroctyes during osteoarthritis.(KTB1100-3.1)
Author:Xiong, Liwei. Publication name: Glycoconjugate Journal IF:3.1
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NCAPD3 is involved in papillary thyroid carcinoma proliferation, metastasis, and aerobic glycolytic pathway.(KTB1100-2.9)
Author:Zhang, Liubing, Aiping Peng, and Yue Qin. Publication name:Discover Oncology IF:2.9
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Microcystin-LR disrupts ovarian granulosa cell glycolysis via GSK3β-Mediated HK2 mitochondrial dissociation: Evidence from integrated In Vivo and In Vitro models(KTB1100-2.8)
Author:J Yuan, X Li, S Yan, C Luo, S Xian, Y Li, J Wu Publication name:JOURNAL OF BIOLOGICAL CHEMISTRY IF:2.8
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Porcine reproductive and respiratory syndrome virus-mediated lactate facilitates virus replication by targeting MAVS(KTB1100-2.7)
Author:L Zhang, X Liu, J Mao, Y Sun, Y Gao, J Bai, ... Publication name:Br J Nutr IF:2.7
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LRRN3 protects dopaminergic neurons by inhibiting glycolysis in Parkinson's disease(KTB1100-2.6)
Author:J Gao, K Qin, W Xian, J Ren, A Xie, B Hou Publication name:Cytotherapy IF:2.6
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Low level of lactate and trophoblast dysfunction mediated by SNAI1/PDK1 contributes to miscarriage.(KTB1100-2.5)
Author:Gou, Rui, et al. Publication name: Placenta IF:2.5
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Revealing the effects of lactate on bovine SCNT embryo development through transcriptome sequencing analyses.(KTB1100-2.5)
Author:Li, Nannan, et al. Publication name:Theriogenology IF:2.5
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Transcription Factor STAT3-Activated LDHB Promotes Tumor Properties of Endometrial Cancer Cells by Inducing MDH2 Expression.(KTB1100-2.5)
Author:Shen, Li, et al. Publication name: Molecular Biotechnology IF:2.5
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Histone H3K18 lactylation-dependent epigenetic activation of RUNX2 orchestrates PI3K/AKT oncogenic signaling in laryngeal squamous cell carcinoma(KTB1100-2.2)
Author:D Fu, X Huang, Y Lin, F Yu, H Wu Publication name: IF:2.2
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Lactate administration induces skeletal muscle synthesis by influencing Akt/mTOR and MuRF1 in non‐trained mice but not in trained mice.(KTB1100-2)
Author:Kyun, Sunghwan, et al. Publication name:Physiological Reports IF:2
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Regulatory effect of lactate on peripheral blood CD4+ T cell subsets in patients with rheumatoid arthritis." Beijing da xue xue bao. Yi xue ban= Journal of Peking University.(KTB1100-2)
Author:Huang, Huina, et al. Publication name:Health Sciences IF:2
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Divalent Metal Cations Favor Bone Regeneration Through Promoting Aerobic Glycolysis of Differentiated Mscs and Modulating Activity of Sensory and Autonomic Nervous Systems.(KTB1100-)
Author:Luo, Ying, et al. Publication name: IF:
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Hypoxia Promotes the In Vitro Proliferation of Buffalo Spermatogonial Cells by Increasing Lactate and H3K18la Lactylation Levels.(KTB1100-)
Author:Li, Mengqi, et al. Publication name:Cells 14.11 IF:
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Via inhibiting glycolysis and oxidative respiration and promoting ferroptosis, ETFDH inhibits the proliferation of colorectal cancer.(KTB1100-)
Author:Feng, Yaoyao, et al. Publication name: IF:



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