Chlamydia pneumonia IgM ELISA Kit

Principle of the Assay: Diluted patient serum (serum diluent contains sorbent to remove rheumatoid factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample.

Intended Uses: Chlamydia Pneumoniae IgM ELISA Kit is intended for the detection of IgM antibody to C. Pneumoniae in human serum or plasma.

1. Verkooyen RP; Van Lent NA; Mousavi Joulandan SA; Snijder RJ; van den Bosch JM; Van Helden HP; Verbrugh HA. Diagnosis of Chlamydia pneumoniae infection in patients with chronic obstructive pulmonary disease by micro-immunofluorescence and ELISA. J Med Microbiol 1997; 46(11):959-642. Eckman MR, Leinonen M, Syrjala H, et al. Evaluation of serological methods in the diagnosis of Chlamydia pneumoniae during an epidemic in Finland. Eur J Clin Microbiol Infect Dis 1993;12:756-60.3. Bourke SJ, Lightfoot NF. Chlamydia pneumoniae: defining the clinical spectrum of infection requires precise laboratory diagnosis. Thorax 1995;50(Suppl 1):543-8.4. Hammerschlag MR, Chirgwin K, Roblin PM, et al. Persistent infection with Chlamydia pneumoniae following acute respiratory illness. Clin Infect Dis 1992;14:178-82.5. Leinonen, M., Sryjala, H., Kujala, P. and Saikku, P. (1991). Serological diagnosis of Chlamydia pneumoniae (Cpn) in adults. In: Abstracts of 31st ICAAC, Chicago, Illinois, Sept 29 - Oct 2. Washington, D.C. Amer. Soc. Microbiol, 209.6. Cook PJ, Honeybourne D. Chlamydia pneumoniae. J Antimicrobial Chemother 1994;34:859-73.7. Nystrom-Rosander C, Thelin S, Hjelm E, et al. High incidence of Chlamydia pneumoniae in sclerotic heart valves of patients undergoing aortic valve replacement. Scan J Infect Dis 1997;29:361-5

Small volumes of Chlamydia pneumonia IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS580094 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Chlamydia pneumonia IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Chlamydia pneumonia IgM. The ELISA analytical biochemical technique of the MBS580094 kit is based on Chlamydia pneumonia IgM antibody-Chlamydia pneumonia IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Chlamydia pneumonia IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Chlamydia pneumonia IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).