Conditionnement : 1g
Téléphone : +1 850 650 7790
Synonyms: Cholesteryl alcohol, Cholesterin
| Description | Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia. |
| In vitro | METHODS: CD4+ T lymphocytes were incubated with 7-KC (17.5-70 µM) and Cholesterol-MβCD (17.5-70 µM) for 10 min, and T cell membrane order and disorder were assessed using di-4 ANEPPDHQ fluorescent dye. RESULTS: After exposure to 7-KC, T cell membrane order was altered in a dose-dependent manner, with significant reconstitution of membrane order observed only in cells treated with 35 µM Cholesterol, while reconstitution with l7.5 µM Cholesterol induced minimal effects. [1] METHODS: Human gastric cancer cells SNU601, SNU638 and SNU216 were treated with Cholesterol (25-100 µM) for 48 h and cell viability was measured using MTT Assay. RESULTS: Cholesterol caused a dose-dependent decrease in cell viability in all three cell lines. [2] |
| In vivo | METHODS: To induce hypercholesterolemia, STD:ddY mice were fed a high cholesterol diet (1% cholesterol, 0.5% cholic acid, 0.5% olive oil and 93% standard mouse chow). RESULTS: Cholesterol can be used to construct a mouse model of hypercholesterolemia. [3] METHODS: To induce hyperlipidemia, CD-1 mice were fed a high cholesterol diet (2% cholesterol and 0.6% sodium deoxycholate). RESULTS: Cholesterol can be used to construct a mouse model of hyperlipidemia. [4] |
| Synonyms | Cholesteryl alcohol, Cholesterin |
| Disease Modeling Protocol | Hyperlipidemia model
*Precautions： *References：Xiangdong L,et,al. Animal models for the atherosclerosis research: a review. Protein Cell. 2011 Mar;2(3):189-201. |
| Molecular Weight | 386.65 |
| Formula | C27H46O |
| Cas No. | 57-88-5 |
| Smiles | C[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)14)14)(CC[C@@]2([C@@H](CCCC(C)C)C)14)14 |
| Relative Density. | 1.06 g/cm3 |
| Storage | Store at low temperature,Store under nitrogen Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||
| Solubility Information | H2O: < 1 mg/mL (insoluble)  DMSO: < 1mg/mL (insoluble) Ethanol: 11.42 mg/mL (29.54 mM), Sonication is recommended. | |||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||
Ethanol
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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