Choline/Acetylcholine Fluorometric Assay Kit
Référence MBS2572183-5x96
Conditionnement : 5x96Tests
Marque : MyBiosource
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Choline/Acetylcholine Assay Kit
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2572183MBS2572183 is an assay kit targeting Choline/Acetylcholine. Also known as Choline/Acetylcholine Fluorometric Assay Kit. More details
Product Overview
Online Datasheet in PDFProduct Name
Choline/Acetylcholine, Assay Kit
Full Product Name
Choline/Acetylcholine Fluorometric Assay Kit
Assay Type
Quantitative
Samples
serum, plasma and animal tissue
Detection Range
0.06-10 umol/L
Sensitivity
0.06 umol/L
Preparation and Storage
This product can be stored at -20 degree Cfor 12 months with shading light.
Ship: Ice bag
Ship: Ice bag
Detection Method
Fluorescence method
Detection Instrument
Fluorescence Microplate reader
Assay Time
30 min
Research Area
Glycolysis and Lipid Metabolism
Test Principle
Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, often grouped with B complex vitamins, that plays a key role in many biological processes. Choline is a precursor to the synthesis of acetylcholine (AchE), an important neurotransmitter in the peripheral and central nervous systems.Choline is measured by enzyme coupling reaction to generate fluorescent products in this kit, and the content of fluorescent substances is proportional to the presence of choline. Acetylcholinesterase was added to the reaction to hydrolyze acetylcholine into choline and acetic acid, and the acetylcholine content was further calculated by measuring the total amount of choline and acetylcholine.
Precision
Inter Assay CV: 8.2-10%
Intra Assay CV: 3.2-5%
Intra Assay CV: 3.2-5%
Product Categories/Family for Choline/Acetylcholine assay kit
Metabolism Assay; Cell Metabolism Assays; Glycolysis and Lipid Metabolism
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of Choline/Acetylcholine assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.