Citric acid [77-92-9]
Référence T5S0636-1ml
Conditionnement : 1mLx10mM(inDMSO)
Marque : TargetMol
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Citric acid
(Synonyms: Citro, Citretten) Copy Product Info
Citric acid (Citro) is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a food additive and a natural preservative.
Cas No. 77-92-9
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:99.94%
Appearance:Solid
Color:White
COA LCMS HNMR
Product Introduction
Citric acid AI Summary
Citric acid exhibits a range of bioactivities and interactions across various biological assays. It demonstrates significant binding energy, with a measured binding energy of 5.0 kCal mol-1, indicating the potential to interact with other molecules. The compound shows weak inhibitory activity against the SH2 domain of human p60 c-Src tyrosine kinase, with an IC50 value greater than 40,000,000.0 nM in a surface plasmon resonance assay. In enzymatic assays, Citric acid exhibits inhibitory activity against rat ATP-Citrate lyase, with a relative Vmax value of 1.0 and a Km value of 110,000.0 nM. It also shows binding affinity for Helicobacter pylori DHQase 2 (Kd of 2500.0 nM) and Streptomyces coelicolor DHQase 2 (Kd of 7300.0 nM), and inhibitory activity for Mycobacterium tuberculosis DHQase 2 at 20 mM with inhibition below 10.0%. The compound lowers plasma glucose levels by 47.3% in Swiss-Webster mice at a dose of 250 mg/kg intraperitoneally after 4 hours, although it also demonstrates toxicity with an LC50 of 50.0 ppm in brine shrimp. It inhibits both Bacillus licheniformis beta-lactamase BS3 and beta-lactamase TEM1 with Ki values of 490,000.0 nM and 730,000.0 nM, respectively, in an acetate buffer at pH 5. Furthermore, Citric acid shows 100.0% HDAC inhibitory activity at 500 uM in a fluorimetric assay and potency in various cellular ATP level measurements, ranging from 2.0 nM to 79,432.8 nM. It also demonstrates activity in receptor signaling pathways, such as glucocorticoid receptor and peroxisome proliferator-activated receptor gamma, and inhibits aldehyde dehydrogenase and lipase co-activator protein ABHD5 interactions. Additionally, it inhibits the uptake of estrone sulfate in OATP-B-expressing HEK293 cells with 97.7% activity and exhibits antioxidant properties with an EC50 of 0.074 ug/mL-1 for Fe2+ chelating activity. The compound also shows inhibition against human hexahistidine-tagged full-length FTO and recombinant ALKBH5 catalytic domain, with IC50 values of 300,000.0 nM and 488,000.0 nM, respectively. Citric acid has an LC50 of 50.0 ppm in brine shrimp toxicity assays and demonstrates a range of biochemical and hematological parameter effects, indicating potential hepatotoxic, nephrotoxic, and hematotoxic impacts. It shows antimicrobial activity against Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, and Staphylococcus aureus, with inhibitory zone sizes ranging from 0.0 mm to 10.67 mm at 40 μg per disc. The compound also possesses antiviral activity, inhibiting SARS-CoV-2 induced cytotoxicity in Caco-2 and VERO-6 cells, as well as showing an IC50 of 212,600.0 nM against Paramecium bursaria chlorella virus 1 CPH. Finally, Citric acid exhibits inhibitory activity on various enzymes, including human ACMSD, HDAC6, PHD1, PHD2, PHD3, KDM5B, and N-terminal His6-tagged recombinant human ALKBH5 catalytic AlkB domain. It demonstrates a lack of significant binding affinity or inhibition in assays targeting DRD1, SLC6A3, ESR1, and other receptors and enzymes at explored concentrations (AC50 > 30000.0 nM). This extensive profile suggests potential therapeutic applications, but also highlights the need for further studies to comprehensively understand its bioactivity and safety profile..
Note: Summary generated by AI. Data source: ChEMBL 
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Citric acid (Citro) is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a food additive and a natural preservative. |
| Targets & IC50 | HaCaT cells:12.5 mM, GF:0.28 per cent |
| In vitro | METHODS: HaCaT cells were treated with Citric acid (0-12.5 mM) for 24 hours, and the percentage of viable cells was determined by flow cytometry. RESULTS: Citric acid exhibited anti-proliferative activity in a dose-dependent manner. [1] METHODS: HaCaT cells were treated with Citric acid (12.5 mM) for 48 hours, and the levels of related proteins were detected by western blot. RESULTS: Citric acid increased the expressions of FAS, BAX, BID, AIF, EndoG, cytochrome C, PARP, GADD153, GRP78 and Caspase-3, -8, -9, and decreased the expressions of BCL-2 and BCL-Xl. [1] |
| In vivo | METHODS: To study the effect of Citric acid on the liver of mice, Citric acid (120, 240, and 480 mg/kg) was intraperitoneally injected into mice. RESULTS: Citric acid significantly reduced the activity of GSH-Px in the liver of mice, induced an increase in the level of MDA (malondialdehyde), and increased the activity of caspase-3 to induce apoptosis in a dose-dependent manner. [2] METHODS: To study the nephrotoxicity of Citric acid, Citric acid (120, 240, and 480 mg/kg) was intraperitoneally injected into mice every week for 3 consecutive weeks. RESULTS: Citric acid causes nephrotoxicity in mice. [3] |
| Synonyms | Citro, Citretten |
| Molecular Weight | 192.12 |
| Formula | C6H8O7 |
| Cas No. | 77-92-9 |
| Smiles | O=C(O)CC(O)(C(=O)O)CC(=O)O |
| Relative Density. | 1.67. Temperature:20 °C. |
| Storage | Keep away from direct sunlight,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | H2O: 100 mg/mL (520.51 mM), Sonication is recommended.  DMSO: 240 mg/mL (1249.22 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (26.03 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
H2O/DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||