Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Référence TA50011-100
Conditionnement : 100ul
Contactez votre distributeur local :
Téléphone :
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
SKU
TA50011-100
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
| Product Data | |
| Clone Name | OTI4C5 (formerly 4C5) |
|---|---|
| Application | FC, IF, IP, WB |
| Recommended Dilution | WB: 1:2000, IP: 1:200, IF 1:1000, FC: 1:1000, IHC: 1:1000-1:2000 |
| Reactivity | DDK (FLAG tag): DYKDDDDK |
| Antibody Host | Mouse |
| Clonality | Monoclonal |
| Immunogen | Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH |
| Isotype | IgG2a |
| Formulation | PBS (pH7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Storage | Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months. |
| Stability | Stable for 1 year at -20°C from date of shipment. |
| Shipping | Blue Ice |
| Synonyms | ANTI DDK, ANTI-DDK, ANTIDDK, FLAG TAG, ANTI FLAG, ANTI-FLAG, ANTI FLAG ANTIBODY, FLAG ANTIBODY, M2 ANTIBODY, FLAG M2, ANTIBODY FOR FLAG, 4C5, ANTI DDK 4C5, DDK 4C5, ANTI-DDK 4C5, DYKDDDDK TAG, D-TAG ANTIBODY, ANTI D-TAG, ANTI D TAG, ANTI-D TAG, ANTI-MDDK, ANTI-MYC DDK, ANTI-MYC/DDK, MYC/DDK ANTIBODY, MYC DDK ANTIBODY, ANTI MYC DDK, ANTI DDK MYC, ANTI-DDK/MYC, ANTI-DDK MYC, FLAG AB, FLAG MAB, DDK ANTIBODY |
| Note | This antibody doesn't recognize N- terminus DDK tag. Please use TA180144 for the detection of DDK tag at any location in Western blotting. |
| Product Manuals |
| FAQs |
|
| SDS |
| Antibody Resources |
|
| SKU | Description | Size | ||
|---|---|---|---|---|
| CF50011 | Carrier-free (BSA/glycerol-free) Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 100 ug | | |
| TA150015 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Biotin | 100 ul | | |
| TA150020 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, Phycoerythrin (PE) | 100 ul | | |
| TA150021 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 488 | 100 ul | | |
| TA150022 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 550 | 100 ul | | |
| TA150030 | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal antibody, HRP | 100 ul | | |
| TA150037 | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads | 1 ml | | |
| TA150037S | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose Beads | 500 ul | | |
| TA150042 | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic Beads | 1 ml | | |
| TA150042S | Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Magnetic Beads | 500 ul | | |
| TA183048 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody, DyLight 594 | 100 ul | | |
| TA50011-1 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 1 ml | | |
| TA50011-30 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 30 ul | | |
| TA50011-5 | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody | 5 ml | | |
Citations
Stabilization of CDK6 by ribosomal protein uS7, a target protein of the natural product fucoxanthinol
Iizumi, Sowa, Goi et al
Commun Biol (2022) 5 (1), 564 DOI: 10.1038/s42003-022-03522-6
Iizumi, Sowa, Goi et al
Commun Biol (2022) 5 (1), 564 DOI: 10.1038/s42003-022-03522-6
Cited by 6
AI Summary: OriGene’s DDK antibody (TA50011-100) was used for immunodetection in this study to analyze protein expression levels, contributing to the investigation of CDK6 stabilization mechanisms.
Snippet: or ab210745, Abcam, Cambridge, UK, 1:500 or 1:1000 dilution), uS9 (ab26159, Abcam, 1:500 dilution), uS4 (ab74711, Abcam, 1:1000 dilution), uL3 (ab241412, Abcam, 1:500 dilution), and DDK (TA50011-100, OriGene Technologies, Rockville, MD, USA, 1:500 dilution). The membranes were then incubated with HRP-linked anti-rabbit IgG (NA934V, GE Healthcare, Chicago, IL, USA, 1:2000 dilution) or HRP-linked an;, Cambridge, UK, 1:500 or 1:1000 dilution), uS9 (ab26159, Abcam, 1:500 dilution), uS4 (ab74711, Abcam, 1:1000 dilution), uL3 (ab241412, Abcam, 1:500 dilution), and DDK (TA50011-100, OriGene Technologies, Rockville, MD, USA, 1:500 dilution). …
Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease
Barbier-Torres, Murray, Yang et al
Nat Commun (2022) 13 (1), 557 DOI: 10.1038/s41467-022-28201-2
Barbier-Torres, Murray, Yang et al
Nat Commun (2022) 13 (1), 557 DOI: 10.1038/s41467-022-28201-2
Cited by 41
AI Summary: OriGene products contributed to this study by providing DDK antibody (TA50011-100) for protein detection and siRNAs targeting human PIN1 (SR303529) and mouse Pin1 (SR403748) for gene knockdown experiments, enabling analysis of protein expression and the functional role of PIN1 in mitochondrial dysfunction associated with alcohol-related liver disease.
Snippet: … Blots were probed with antibodies diluted 1:1000 in 5% skim milk against MATα1 (ab129176), PIN1 (07-091, Millipore), TOM20 (ab78547), DDK (TA50011-100, Origene), His-Tag (A00186-100, GenScript), OXPHOS (ab110413), CK2α (2656, Cell Signaling), CPT1α (12252, Cell Signaling), SDHα (5839, Cell Signaling), MCAD (ab110296), JNK (9252, Cell Signaling), p;- For PIN1 knockdown 50 nM siRNA against human PIN1 (SR303529, Origene) or mouse Pin1 (SR403748, Origene) or equivalent scramble control (SC) were delivered
An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury
Hilton, Husch, Schaffran et al
Neuron (2021) 110 (1), 51-69.e7 DOI: 10.1016/j.neuron.2021.10.007
Hilton, Husch, Schaffran et al
Neuron (2021) 110 (1), 51-69.e7 DOI: 10.1016/j.neuron.2021.10.007
Cited by 130
AI Summary: In this study, the Mouse anti-DDK (FLAG) antibody from OriGene (TA50011-100) was used to detect FLAG-tagged proteins, aiding in the investigation of synaptic vesicle priming proteins' roles in axon regeneration after CNS injury.
Snippet: 140 103;RRID: AB_88776 Rabbit anti-Glial Fibrillary Acidic Protein (GFAP) DAKO Z0334;RRID: AB_10013382 Rabbit anti-RFP Rockland 600-401-379;RRID: AB_2209751 Mouse anti-DDK (FLAG) Origene TA50011-100;RRID: AB_2622345 Guinea Pig anti-NeuN Merck Millipore ABN90P;RRID: AB_2341095 Donkey anti-chicken IgY IgG H+L Alexa Fluor 488 Jackson Immunoresearch 703-545-155;RR
EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence
Gautheron, Morisseau, Chung et al
Elife (2021) 10 DOI: 10.7554/eLife.68445
Gautheron, Morisseau, Chung et al
Elife (2021) 10 DOI: 10.7554/eLife.68445
AI Summary: OriGene’s Anti-Flag antibody (Cat# TA50011-100) was used in this study for Western blot, immunofluorescence, and immunoprecipitation experiments to detect Flag-tagged proteins and analyze the effects of EPHX1 mutations.
Snippet: Antibody Anti-Flag Origene Cat# TA50011-100 WB (1:1000) - IF (1:1000) - IP (1:200)
α-TubK40me3 is required for neuronal polarization and migration by promoting microtubule formation
Xie, Wang, Li et al
Nat Commun (2021) 12 (1), 4113 DOI: 10.1038/s41467-021-24376-2
Xie, Wang, Li et al
Nat Commun (2021) 12 (1), 4113 DOI: 10.1038/s41467-021-24376-2
Cited by 32
AI Summary: OriGene's Flag antibody (TA50011-100) was used in this study to detect Flag-tagged proteins, facilitating the analysis of protein expression related to α-TubK40me3’s role in neuronal polarization and migration.
Snippet: iton X-100 against α-tubulin (1:10000;Sigma T5168), SETD2 (1:1000;Sigma HPA042451), α-TubK40me3 (1:1000;homemade), GFP (1:2000;Abcam ab13970), SMI-312 (1:500, Biolegend 837904) or Flag (1:1000;Origene TA50011-100) overnight at 4 °C. The samples were then incubated with corresponding Alexa-Fluor-conjugated secondary antibodies (1:1000;Molecular probe) and DAPI (1:2000;Sigma) for 60 mi
Axon-enriched lincRNA ALAE is required for axon elongation via regulation of local mRNA translation
Wei, Huang, Li et al
Cell Rep (2021) 35 (5), 109053 DOI: 10.1016/j.celrep.2021.109053
Wei, Huang, Li et al
Cell Rep (2021) 35 (5), 109053 DOI: 10.1016/j.celrep.2021.109053
Cited by 28
AI Summary: OriGene’s Mouse monoclonal anti-FLAG antibody (Cat#: TA50011-100) was used in this study to detect FLAG-tagged proteins, facilitating the investigation of molecular interactions involved in axon elongation.
Snippet: Mouse monoclonal anti-FLAG Origene Cat#: TA50011-100;RRID: AB_2622345
Showing 1-6 of 28 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Related Products
Vous serez peut-être également intéressé par les produits suivants :
Référence
Description
Cond.
Prix HT