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> Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen) (APC)

Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen) (APC)

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Référence C7510-50H-APC-100ul

Conditionnement : 100ul

Marque : US Biological

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Téléphone : +1 850 650 7790

C7510-50H-APC Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Basement Membrane Alpha 2 Chain, Collagen Type IV Alpha 1, Collagen Type IV Alpha 2, DKFZp686I14213, FLJ22259, Goodpasture antigen) (APC)

Clone Type
Polyclonal
Host
goat
Source
human
Swiss Prot
P02462
Isotype
IgG
Grade
Affinity Purified
Applications
DB FLISA IC IHC
Crossreactivity
Bo Hu Mo
Accession #
NM_001845.4
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:
Suitable for use in Dot and Slot blot, FLISA, Immunohistochemistry, and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
*Dot and Slot blot: 1:100-1:500
FLISA: 1:1000-1:4000
*Immunohistochemistry (Frozen): 1:10-1:40
Immunocytochemistry: 1:10-1:20
Optimal dilutions to be determined by the researcher.

*Note: Harsh chemicals or procedures involving heat may denature collagen epitopes. We recommend avoiding such actions when using this antibody.

Positive Controls:
Kidney, muscle, tendon spleen tissue

Negative Control:
Neurons/glia

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by affinity chromatography|Immunogen: Human and bovine placental collagen type IV|Specificity: Recognizes the conformational determinants on human type IV collagen. Less than 10% crossreactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). Species Crossreactivity: bovine and mouse||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Human and bovine placental collagen type IV
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by affinity chromatography
Specificity
Recognizes the conformational determinants on human type IV collagen. Less than 10% crossreactivity with collagen types I, II, III, V and VI. May show reactivity to type IV collagen from other species. Has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin). Species Crossreactivity: bovine and mouse
References
1. Marneros, A. G. et al., EMBO J. 23: 89-99, (2004). 2 Myers, J. C. et al. Experimental Cancer Research 253: 587–598.(1999).

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