Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1

COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1

Imprimer Imprimer
Identifiez-vous

Référence IEQ-CoVS1RBDA1-IgG-2

Conditionnement : 2PlateKit

Marque : RayBiotech

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

COVID-19 Alpha Spike Variant Human IgG ELISA Kit Standard CurveExtrapolated antibody concentrations (Units/mL) for a single sample tested at four dilutions across three assay runs.COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1

COVID-19 Alpha Spike Variant Human IgG ELISA Kit Standard Curve

COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1

The RayBiotech COVID-19 Alpha Spike Variant Human IgG ELISA Kit 1 is an antibody detection kit (indirect ELISA method) for the quantitative measurement of human IgG against the spike protein mutations (S494P) associated with the Alpha variant of SARS-CoV-2. This Product is for Research Use Only. Not for use in in vitro diagnostics.
Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Detection Range
1.37 Units/ml - 1000 Units/ml
Compatible Sample TypesSerum
Recommended Dilution (Serum/Plasma)
500-fold
Gene Symbols
IGG
Quantitative/Semi-QuantitativeSemi-Quantitative
Solid Support
96-well Microplate
Design PrincipleIndirect
Method Of DetectionColorimetric
Research AreaInfectious Disease, COVID-19
Shipping TypeDry ice (additional shipping fee)
Storage-80°C
NoteThis Product is for Research Use Only, not for use in in vitro diagnostics, following guidance from the FDA for tests submitted to the FDA for Emergency Use Authorization and Notification on March 16th, 2020, and amended on May 4th, 2020. While this test has been submitted for FDA EUA Authorization, it currently has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status. Please consult the manual for specific details and limitations of this test.

Kit Components

  • SARS-CoV-2 Protein coated 96-well microplate
  • Wash Buffer
  • Positive Control
  • Biotinylated Anti-Human IgG
  • HRP-Streptavidin concentrate
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diluent
  • ample Diluent

Additional Materials Required

  • Microplate reader capable of measuring absorbance at 450 nm
  • Precision pipettes to deliver 2 µL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Distilled or deionized water

Assay Procedure Summary

  1. Prepare all reagents, samples and standards as instructed.
  2. Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
  3. Add 100 µL prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
  4. Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
  5. Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
  6. Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

citations

  • Emerging Technologies of Three-Dimensional Printing and Mobile Health in COVID-19 Immunity and Regenerative Dentistry

    Year:
    2023
    Journal Name:
    Tissue Engineering Part C-methods