RayBio® COVID-19 N and S1 RBD protein Human Antibody Detection Kit (Indirect ELISA method) for the quantitative measurement of human IgG antibody against SARS-CoV-2 in serum. This Product is for Research Use Only. Not for use in in vitro diagnostics.
IEQ-CoVS1RBD-IgG1 was used to quantify anti-S1 receptor-binding domain IgG antibodies in plasma samples from cancer patients with COVID-19. Figure adapted from Remsik et al., Cancer Cell, 2021.
COVID-19 Human IgG ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Compatible Sample Types | Plasma, Serum, Dried Blood, Saliva |
| Gene Symbols | IGG |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -80°C |
| Note | This Product is for Research Use Only, not for use in in vitro diagnostics, following guidance from the FDA for tests submitted to the FDA for Emergency Use Authorization and Notification on March 16th, 2020, and amended on May 4th, 2020. While this test has been submitted for FDA EUA Authorization, it currently has not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 infection or to inform infection status. Please consult the manual for specific details and limitations of this test. |
| Antigen | N and S (S1RBD), N-Protein, S-Protein (S1RBD) |
Typical Data
Kit Components
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
- 1X PBS (DBS version)
- Tween-20 (DBS version)
- Protease Inhibitor Cocktail (DBS version)
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Distilled or deionized water
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 μl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
- Add 100 μl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.
documents
citations
Inflammatory Leptomeningeal Cytokines Mediate COVID-19 Neurologic Symptoms in Cancer Patients
- Species:
- Human
- Sample Type:
- Plasma, CSF (Inflammation in the brain during covid)
- Year:
- 2021
- Journal Name:
- Cancer Cell
- PubMed ID:
- 33508216
Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics
- Sample Type:
- Magnetic nanoparticles (MNPs)
- Year:
- 2022
- Journal Name:
- Nature Communications
Vertical Transmission of SARS-CoV-2-Specific Antibodies and Cytokine Profiles in Pregnancy
- Year:
- 2024
- Journal Name:
- Journal of Infectious Diseases
- PubMed ID:
- 37786979
A multiantigenic Orf virus-based vaccine efficiently protects hamsters and nonhuman primates against SARS-CoV-2
- Year:
- 2024
- Journal Name:
- NPJ vaccines
- PubMed ID:
- 39414789
Severe acute respiratory syndrome coronavirus 2-reactive salivary antibody detection in South Carolina emergency healthcare workers, September 2019-March 2020
- Year:
- 2024
- Journal Name:
- Epidemiology and Infection
- PubMed ID:
- 39320488
N-glycosylation of the SARS-CoV-2 spike protein at Asn331 and Asn343 is involved in spike-ACE2 binding, virus entry, and regulation of IL-6
- Year:
- 2024
- Journal Name:
- Microbiology and Immunology
- PubMed ID:
- 38444370
CD8+ T cell memory induced by successive SARS-CoV-2 mRNA vaccinations is characterized by shifts in clonal dominance
- Year:
- 2024
- Journal Name:
- Cell Rep
- PubMed ID:
- 38458195
Vertical Transmission of SARS-CoV-2–Specific Antibodies and Cytokine Profiles in Pregnancy
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Journal of Infectious Diseases
Refocus on Immunogenic Characteristics of Convalescent COVID-19 Challenged by Prototype SARS-CoV-2
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Vaccines
Exosome-Based Multivalent Vaccine: Achieving Potent Immunization, Broadened Reactivity, and Strong T-Cell Responses with Nanograms of Proteins
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2023
- Journal Name:
- Microbiology spectrum
