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> Cremophor EL-40 [61791-12-6]

Cremophor EL-40 [61791-12-6]

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Référence HY-Y1890-100mL

Conditionnement : 100mL

Marque : MedChemExpress

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Description

Cremophor EL-40, a polyoxyethylene castor oil derivative, is a nonionic surfactant. Cremophor EL-40 is widely employed to improve dissolution and delivery of agents[1].

In Vitro

Cremophor EL-40 is a widely used excipient. In the first 10 h, Cremophor EL-40 at 5 mg/mL increases the impedance, then decreases it, and after 20 h of treatment, epithelial cell death is detected. Cremophor EL-40 decreases cell viability and increases cell toxicity more than RH40. Both epithelial viability and monolayer integrity are seriously impaired at 10 to 50 mg/mL concentrations of Cremophor EL-40 at 24-h treatment. Cremophor EL-40 also enhances cell detachment[1].
Note:
Due to the nature of the product, it may solidify after being stored at 4°C. It can be heated in a 37°C water bath before use. It can be used normally after it is completely dissolved.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cremophor EL-40 Related Antibodies

Solvant et solubilité
In Vitro: 

H2O : 133.33 mg/mL (Need ultrasonic)

Ethanol : ≥ 100 mg/mL

*"≥" means soluble, but saturation unknown.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL; Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Pureté et documentation
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