Cyclin D1 (CCND1) Mouse Monoclonal Antibody [Clone ID: 5D4]
Specifications
Product Data | |
Clone Name | 5D4 |
Applications | FC, IHC, IP, WB |
Recommended Dilution | Western blot: 1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200 μg of cell extract from 5x106 cells. Immunohistochemstry on frozen sections: 1-10 μg/mL; Heat treatment is necessary for paraffin embedded sections. Microwave oven; 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5). Flow cytometry: 10 μg/mL (final concentration). For details see protocol below. |
Reactivities | Human, Mouse |
Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Recombinant human PRAD1/Cyclin D1 |
Specificity | This antibody reacts with human Cyclin D1 (36 kDa). This clone 5D4 recognizes human Cyclin D1, D2 and mouse Cyclin D1, D2, but not human and mouse Cyclin D3. |
Formulation | PBS containing 50% glycerol, pH 7.2 State: Azide Free State: Liquid Ig fraction without preservatives |
Concentration | lot specific |
Purification | Protein A agarose |
Conjugation | Unconjugated |
Storage | Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | cyclin D1 |
Database Link | |
Background | Cyclin D1, also known as Bcl-1, CCND1, or PRAD-1, is a 36 kDa nuclear protein that plays a key regulatory role during the G1 phase of the cell cycle by regulating the activity of Cdk proteins. Cyclin D1 forms a complex with Cdk4, then binds to and phosphorylates Rb protein, triggering cells to progress from G0/G1 to S and thus driving cellular proliferation. Cyclin D1 protein overexpression is found in the majority of human breast cancers and is usually linked with poor prognosis. Overexpression of cyclin D1 is a well-established criterion for the diagnosis of Mantle Cell Lymphoma, and also has been used to distinguish malignant breast carcinomas from premalignant breast lesions. |
Synonyms | Cyclin-D1, PRAD-1 oncogene, BCL-1 oncogene, CCND1, BCL1, PRAD1 |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blottin Immunoprecipitation 1) Deparaffinize the sections with Xylene 3 times for 3-5 minutes each. Flow cytometric analysis for cells
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