Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), Recombinant, Human, His-Tag

Source:
Recombinant protein corresponding to Gln359~Ser858 from human Cystic Fibrosis Transmembrane Conductance Regulator, Intestinal, fused to His-Tag at N-terminal, expressed in E. coli.

Molecular Weight:
~60.3kD

Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 . Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37ºC for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.