D-Lin-MC3-DMA [1224606-06-7]
Référence T5823-10mg
Conditionnement : 10mg
Marque : TargetMol
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
D-Lin-MC3-DMA
Copy Product Info
D-Lin-MC3-DMA is a siRNA delivery vector, an ionizable amino-lipid compound. D-Lin-MC3-DMA offers advantages of high loading efficiency, low toxicity, and efficient endosomal escape.
Cas No. 1224606-06-7
With extensive experience in compound synthesis, we can provide rapid custom synthesis services for this product according to your research needs.
For research use only—not for human use. No sales to individuals. Use as intended only.
Select Batch
Purity:99.86%
COA HNMR LCMS
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | D-Lin-MC3-DMA is a siRNA delivery vector, an ionizable amino-lipid compound. D-Lin-MC3-DMA offers advantages of high loading efficiency, low toxicity, and efficient endosomal escape. |
| Targets & IC50 | FVII protein:0.03 mg/kg |
| In vitro | Replacement of DSPC by unsaturated PCs and DLin-MC3-DMA by the related lipid DLin-KC2-DMA resulted in highly potent transfection reagents for HeLa cells in vitro. |
| Cell Research | White leghorn chicken embryos at stage 24 (30) were removed and dissected in Hanks buffered saline solution (HBSS).?Forelimbs were pooled and treated for 1 h in dispase to remove epithelial ectoderm.?Limb mesenchyme was dissociated, and resuspended in DMEM with 5% FBS and 1% penicillin/streptomycin.?Cells were plated onto 96-well plates at a density of 2x10^5 or 2x10^7 cells/ml and treated with 0-40 μg/ml LNP-pDNA.?Cultures were incubated overnight at 37℃ and resuspended in PBS buffer for expression analysis using a BD LSRII flow cytometer.?Cell survival was assessed in low-density cultures after 24 h of treatment, by counting the adherent cells remaining after PBS washes.?Viability was determined based on average counts of all adherent cells within a single field of view (100x) normalized to cell counts of untreated cultures. |
| Molecular Weight | 642.093 |
| Formula | C43H79NO2 |
| Cas No. | 1224606-06-7 |
| Smiles | O=C(OC(CCCCCCCC/C=C\C/C=C\CCCCC)CCCCCCCC/C=C\C/C=C\CCCCC)CCCN(C)C |
| Relative Density. | 0.886 g/cm3 (Predicted) |
| Storage | Keep away from moisture,Store at low temperature,Keep away from direct sunlight Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||||||||||||
| Solubility Information | Ethanol: 16.67 mg/mL (25.96 mM), Sonication and heating are recommended.  DMSO: 43.33 mg/mL (67.48 mM), Sonication is recommended. | |||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2.00 mg/mL (3.11 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | |||||||||||||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||||||||||||
Ethanol/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||||||||||||