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> D-α-Tocopherol acetate [58-95-7]

D-α-Tocopherol acetate [58-95-7]

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Référence HY-B1278-50g

Conditionnement : 50g

Marque : MedChemExpress

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Description

D-α-Tocopherol acetate (D-Vitamin E acetate) can be hydrolyzed to d-alpha-tocopherol (VE) and absorbed in the small intestine[1].

Cellular Effect
Cell Line Type Value Description References
MCF7 IC50
>500 μM
Compound: 2 (alpha-TOAc)
Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay
Compound was tested for antiproliferative activity against human breast cancer cell line MCF-7 in MTT assay
[PMID: 9873556]
In Vitro

α-Tocopherol acetate (5-30 μg/mL, 24 h) inhibits LPS (HY-D1056)-induced production of NO, prostaglandin E2 (HY-101952) and pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β and IL-6) in mouse peritoneal macrophages[2].
α-Tocopherol acetate (30 μg/mL, 24 h) exhibits a slight suppression on the activation of NF-κB p50 and p65[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

D-α-Tocopherol acetate Related Antibodies

Solvant et solubilité
In Vitro: 

DMSO : 200 mg/mL (423.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 100 mg/mL (211.53 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1153 mL 10.5766 mL 21.1533 mL
5 mM 0.4231 mL 2.1153 mL 4.2307 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (10.58 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.75 mg/mL (5.82 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.75 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (27.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Pureté et documentation
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