DCT Antibody (N-term)

Référence MBS9206412-0,08mL

Conditionnement : 0.08mL

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DCT Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9206412

MBS9206412 is an antibody targeting DCT. Also known as DCT Antibody (N-term). Reactive in Human. Host: Rabbit. DCT is involved in regulating eumelanin and phaeomelanin levels. More details

Product Overview

Online Datasheet in PDF
Product Name
DCT, Polyclonal Antibody
Full Product Name
DCT Antibody (N-term)
Product Gene Name
anti-DCT antibody
Product Synonym Names
L-dopachrome tautomerase; DCT; DT; L-dopachrome Delta-isomerase; Tyrosinase-related protein 2; TRP-2; TRP2; DCT; TYRP2
Clonality
Polyclonal
Isotype
Rabbit IgG
Clone Number
RB23480
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Specificity
This DCT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the N-terminal region of human DCT.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
DCT peptide (MBS9227838) is used for blocking the activity of DCT antibody (MBS9206412)
Immunogen Sequence Positions
62-89
OMIM
191275
3D Structure
ModBase 3D Structure for P40126
Function
Plays a role in melanin biosynthesis (PubMed:33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2- carboxylic acid (DHICA).
Cellular Location
Melanosome membrane; Single-pass type I membrane protein. Melanosome {ECO:0000250|UniProtKB:P29812}. Note=Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38 {ECO:0000250|UniProtKB:P29812}

Product Categories/Family for anti-DCT antibody

Cell biology; Developmental biology; Metabolism
Applications Tested/Suitable for anti-DCT antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC)
Application Notes for anti-DCT antibody
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50

Western Blot (WB) of anti-DCT antibody

Western blot analysis of DCT Antibody (N-term) in K562(lane 1), A375(lane 2), Ramos(lane 3) cell line lysates (35ug/lane). DCT (arrow) was detected using the purified Pab.
Rabbit Anti-DCT Polyclonal Antibody – Cat# MBS9206412

Immunohistochemistry (IHC) of anti-DCT antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with DCT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-DCT Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9206412

Flow Cytometry (FC/FACS) of anti-DCT antibody

DCT Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-DCT Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9206412

Product References and Citations for anti-DCT antibody

Nishihira,J., et.al., Biochem. Biophys. Res. Commun. 243 (2), 538-544 (1998)

Other Notes

Small volumes of anti-DCT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DCT. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-dopachrome tautomerase isoform 2
NCBI Official Synonym Full Names
dopachrome tautomerase
NCBI Official Symbol
DCT
NCBI Official Synonym Symbols
TRP-2; TYRP2
UniProt Protein Name
L-dopachrome tautomerase
UniProt Synonym Protein Names
L-dopachrome Delta-isomerase; Tyrosinase-related protein 2; TRP-2; TRP2
Protein Family
L-dopachrome tautomerase
UniProt Gene Name
DCT
UniProt Synonym Gene Names
TYRP2; DCT; DT; TRP-2; TRP2
UniProt Entry Name
TYRP2_HUMAN
NCBI Protein Information
L-dopachrome tautomerase

UniProt Comments for DCT

DCT: Involved in regulating eumelanin and phaeomelanin levels. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; EC 5.3.3.12; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Isomerase

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: melanosome membrane; melanosome; integral to membrane; cytosol

Molecular Function: copper ion binding; oxidoreductase activity; dopachrome isomerase activity

Biological Process: neuroblast division in the ventricular zone; positive regulation of neuroblast proliferation; epidermis development; pigmentation during development; melanin biosynthetic process from tyrosine; cell development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DCT antibody

 Reference Product  PubMed Publications
 TYRP1 antibody  >68 publications with DCT and TYRP1
 MITF antibody  >56 publications with DCT and MITF
 PMEL antibody  >10 publications with DCT and PMEL
 GAPDH antibody  >5 publications with DCT and GAPDH
 OTX2 antibody  >2 publications with DCT and OTX2
 DDT antibody  >1 publications with DCT and DDT
 SOX9 antibody  >1 publications with DCT and SOX9

Diseases associated with anti-DCT antibody

 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >54 publications with DCT and Kidney Diseases
 Carcinoma Antibodies  >46 publications with DCT and Carcinoma
 Heart Diseases Antibodies  >41 publications with DCT and Heart Diseases
 Neoplasms, Experimental Antibodies  >36 publications with DCT and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >23 publications with DCT and Adenocarcinoma
 Anemia Antibodies  >17 publications with DCT and Anemia
 Lung Neoplasms Antibodies  >17 publications with DCT and Lung Neoplasms
 Necrosis Antibodies  >17 publications with DCT and Necrosis
 Heart Failure Antibodies  >16 publications with DCT and Heart Failure
 Hypertrophy Antibodies  >15 publications with DCT and Hypertrophy

Organs/Tissues associated with anti-DCT antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >288 publications with DCT and Kidney
 Eye Antibodies  >167 publications with DCT and Eye
 Skin Antibodies  >91 publications with DCT and Skin
 Heart Antibodies  >73 publications with DCT and Heart
 Brain Antibodies  >65 publications with DCT and Brain
 Bone Antibodies  >49 publications with DCT and Bone
 Lung Antibodies  >42 publications with DCT and Lung
 Embryonic Tissue Antibodies  >23 publications with DCT and Embryonic Tissue
 Intestine Antibodies  >18 publications with DCT and Intestine
 Connective Tissue Antibodies  >13 publications with DCT and Connective Tissue