Dengue Virus IgG ELISA Kit

Principle of the Assay: Diluted patient serum and biotinylated anti-human IgG antibody reagent are added to the wells coated with Streptavidin. IgG antibodies in the patient serum binds to the x-h IgG antibody, and the complex is immobilized on the plate through streptavidin-biotin interactions. Unbound proteins are washed off through a washing step. Dengue antigen-HRP conjugate reagent is added into the wells. Dengue virus IgG specific antibody, if present, binds to the antigen. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the oxidation of the substrate by the enzyme. The intenSity of the color generated is proportional to the amount of IgG specific antibody in the sample.

Intended Uses: Dengue virus IgG ELISA Kit is intended for the detection of IgG antibody to Dengue virus in human serum or plasma. For research use only. Not for use in test procedures.

1. Pinheiro FP, Corber SJ: Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas. World Health Stat Q 50(3/4):161-169, 1997. 2. Gubler DJ, Trent DW: Emergence of epidemic dengue/dengue hemorrhagic fever as a public health problem in the Americas. Infect Agents Dis 2:383-393, 1993. 3. Wu SJ; Hanson B; Paxton H; Nisalak A; Vaughn DW; Rossi C; Henchal EA; Porter KR; Watts DM; Hayes CG. Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virus. Clin Diagn Lab Immunol1997; 4(4):452-7.4. Lam SK; Devine PL. Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produced during dengue infection. Clin Diagn Virol 1998;10(1):75-8. 5. Rossi CA; Drabick JJ; Gambel JM; Sun W; Lewis TE; Henchal EA. Laboratory diagnosis of acute dengue fever during the United Nations Mission in Haiti, 1995-1996. Am J Trop Med Hyg 1998;59(2):275-8

Small volumes of Dengue Virus IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS580100 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dengue Virus IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Dengue Virus IgG. The ELISA analytical biochemical technique of the MBS580100 kit is based on Dengue Virus IgG antibody-Dengue Virus IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Dengue Virus IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Dengue Virus IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).