Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Digitonin [11024-24-1]

Digitonin [11024-24-1]

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Référence orb1305114-50mg

Conditionnement : 50mg

Marque : Biorbyt

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Digitonin

SKU: orb1305114

Description

Digitonin is a glycoside detergent widely used in research to permeabilize cell membranes via cholesterol binding. Its membrane-disrupting properties facilitate studies in cell biology and biochemistry, both in vitro and in vivo, and it has been investigated for its antitumor potential.

Images & Validation

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Digitonin

Key Properties

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CAS Number11024-24-1
MW1229.31
Purity98.53% (May vary between batches)
FormulaC56H92O29
SMILES00[C@@]12O[C@]3(CC[C@@H](C)CO3)[C@@H](C)[C@]1(00)[C@@]1(C)CC[C@@]3(00)[C@@](00)(CC[C@@]4(00)C[C@@H](O[C@]5(00)O[C@H](CO)[C@H](O[C@]6(00)O[C@H](CO)[C@@H](O)[C@H](O[C@]7(00)OC[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O[C@]6(00)O[C@H](CO)[C@H](O)[C@H](O[C@]7(00)O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O)[C@H](O)[C@H]5O)[C@H](O)C[C@]34C)[C@]1(00)[C@@H]2O
TargetOthers
SolubilityH2O:99.57 mg/mL (81 mM);DMSO:242.5 mg/mL (197.27 mM)

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

1 Results
Dilution
Sample
Summary
Cell culture
Human
20 μg/mL
Human HeLa cells
Digitonin (20 μg/mL) was used in...

Bioactivity

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Target IC50
LLC cells:17.2 µM|CT-26 cells:5 µM|4T1 cells:7.8 µM|RBC membranes (sheep):15.1 µM|MC38 cells:4.3 µM|Caco-2 cells:15.72 ± 0.16 µM|MCF-7 cells:5.23 ± 0.61 µM
In Vivo
METHODS: To determine whether Digitonin enhances the efficacy of CBDCA in vivo, Digitonin (0.2 mL 400 μM) was hepatic arterially infused into LH rats bearing hepatocellular carcinoma tumors, followed by injection of CBDCA 10 min later. RESULTS: Digitonin pretreatment increased the tumor uptake of CBDCA and enhanced the cytotoxic effect of CBDCA.
In Vitro
METHODS: Rat adrenal pheochromocytoma cells PC-12 were treated with Digitonin (10 μg/mL) for 10 min to increase cell permeability. RESULTS: After permeabilization with Digitonin, PC12 cells could be stimulated to release norepinephrine by micromolar concentrations of free Ca2+. METHODS: Human glioblastoma cells U87 were treated with Digitonin (1-5 μg/mL) for 20 min, and cell viability was measured by MTT after overnight cell incubation. RESULTS: 1 μg/mL Digitonin treatment resulted in a slight increase in cell viability, 3 μg/mL Digitonin reduced the growth-promoting effect, and the cell viability was similar to that of the control, and 5 μg/mL Digitonin inhibited the cell viability, which indicated a significant toxicity to the cells. METHODS: Immortalized human T-lymphocytes Jurkat were treated with Digitonin (1-4 μg/mL) for 20 min and cell viability was measured by Resazurin assay. RESULTS: Digitonin treatment resulted in a significant decrease in metabolic activity and this inhibition was concentration-dependent.

Storage & Handling

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Storage-20°C
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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cell, cholesterol, detergent, Digitonin, Inhibitor, inhibit, molecules, mild, permeability
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  • Digitonin

    Digitonin [orb508661]

    > 98%

    11024-24-1

    1229.3

    C56H92O29

    20 mg
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    MRL-SYKi [orb3173401]

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  • U73122

    U73122 [orb1226956]

    >98% (HPLC)

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    464.64

    C29H40N2O3

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  • Digitonin

    Digitonin [orb1226983]

    >98% (HPLC)

    11024-24-1

    1229.34

    C56H92O29

    25 mg, 100 mg, 200 mg, 500 mg, 1 g

Compound Identifiers

PubChem CID
6474107 ↗

Key Properties

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Protocol Information