In cryobiology DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used.

Synonyms:
Methyl Sulfoxide; Dimethylsulfoxide; DMSO

CAS No:
67-68-5

Molecular Formula:
C2H6SO

Molecular Weight:
78.13

Purity:
≥99%

Appearance:
Colorless, clear liquid at RT

Specific Gravity:
1.095-1.097

Boiling Point:
188-190°C

Congealing Temp:
18.3°C

Refractive Index:
1.4755-1.4775

pKa:
31.3

Water (KF):
≤0.1%

Titratable Acid:
≤5ml

Residue after Evaporation:
≤0.01%

Solubility:
Water (very soluble)

RNase:
None Detected

DNase:
None Detected

Storage and Stability:
Store at RT. Stable for 12 months at RT after receipt.

Note:
DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state.