DNMT3L siRNA (Human)
Référence MBS8212962-5x30nmol
Conditionnement : 5x30nmol
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DNMT3L siRNA – DNA (Cytosine-5)-Methyltransferase 3-Like Isoform 1 (Human)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8212962MBS8212962 is an siRNA targeting DNMT3L. Also known as DNMT3L siRNA (Human). Reactive in Human. Host: Synthetic. siRNA to inhibit DNMT3L expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
DNMT3L, siRNA
Full Product Name
DNMT3L siRNA (Human)
Product Gene Name
DNMT3L sirna
Product Synonym Names
DNA; cytosine-5)-methyltransferase 3-like
Host
Synthetic
Species Reactivity
Human
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
DNMT3L siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
43,670 Da
OMIM
606588
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DNMT3L gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for DNMT3L sirna
RNA Interference (RNAi)
Other Notes
Small volumes of DNMT3L sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for DNMT3L. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 3-like isoform 1
NCBI Official Synonym Full Names
DNA (cytosine-5-)-methyltransferase 3-like
NCBI Official Symbol
DNMT3L
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 3-like
Protein Family
DNA (cytosine-5)-methyltransferase
UniProt Gene Name
DNMT3L
UniProt Entry Name
DNM3L_HUMAN
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 3-like
NCBI Summary for DNMT3L
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Show more UniProt Comments for DNMT3L
DNMT3L: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3. Belongs to the C5-methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: cytosol; nucleus
Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine; regulation of gene expression, epigenetic
Show more Protein type: Amino Acid Metabolism - cysteine and methionine; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: cytosol; nucleus
Molecular Function: protein binding; enzyme binding; metal ion binding; enzyme activator activity
Biological Process: positive regulation of catalytic activity; genetic imprinting; DNA methylation; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine; regulation of gene expression, epigenetic
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with DNMT3L sirna
| Reference Product | PubMed Publications |
|---|---|
| DNMT3A sirna | >120 publications with DNMT3L and DNMT3A |
| DNMT3B sirna | >90 publications with DNMT3L and DNMT3B |
| DNMT1 sirna | >52 publications with DNMT3L and DNMT1 |
| HDAC1 sirna | >6 publications with DNMT3L and HDAC1 |
| TRDMT1 sirna | >5 publications with DNMT3L and TRDMT1 |
| TDG sirna | >2 publications with DNMT3L and TDG |
Pathways associated with DNMT3L sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| DNA Methylation Pathway antibodies | DNA Methylation Pathway Diagram |
| Epigenetic Regulation Of Gene Expression Pathway antibodies | Epigenetic Regulation Of Gene Expression Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Trans-sulfuration And One Carbon Metabolism Pathway antibodies | Trans-sulfuration And One Carbon Metabolism Pathway Diagram |
Diseases associated with DNMT3L sirna
| Disease Name | Pubmed Publications |
|---|---|
| Male Urogenital Diseases Antibodies | >14 publications with DNMT3L and Male Urogenital Diseases |
| Genital Diseases, Male Antibodies | >14 publications with DNMT3L and Genital Diseases, Male |
| Infertility, Male Antibodies | >13 publications with DNMT3L and Infertility, Male |
| Congenital Abnormalities Antibodies | >6 publications with DNMT3L and Congenital Abnormalities |
| Genital Diseases, Female Antibodies | >6 publications with DNMT3L and Genital Diseases, Female |
| Infertility, Female Antibodies | >5 publications with DNMT3L and Infertility, Female |
| Cell Transformation, Neoplastic Antibodies | >4 publications with DNMT3L and Cell Transformation, Neoplastic |
| Oligospermia Antibodies | >3 publications with DNMT3L and Oligospermia |
| Prenatal Exposure Delayed Effects Antibodies | >3 publications with DNMT3L and Prenatal Exposure Delayed Effects |
| Breast Neoplasms Antibodies | >2 publications with DNMT3L and Breast Neoplasms |
Organs/Tissues associated with DNMT3L sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Testis Antibodies | >29 publications with DNMT3L and Testis |
| Embryonic Tissue Antibodies | >21 publications with DNMT3L and Embryonic Tissue |
| Placenta Antibodies | >9 publications with DNMT3L and Placenta |
| Mixed Antibodies | >1 publications with DNMT3L and Mixed |
| Epididymis Antibodies | >1 publications with DNMT3L and Epididymis |