Dog Osteocalcin (OC) ELISA Kit
Référence MBS2701833-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Osteocalcin (OC) ELISA Kit for Dog
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2701833SPECIAL PROMOTIONAL PRICING for a limited time
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of osteocalcin in canine serum, plasma, tissue homogenates and other biological fluids.Principle of the Assay: The microplate provided in this kit has been… More details
Product Overview
Online Datasheet in PDFProduct Name
Osteocalcin (OC), ELISA Kit
Full Product Name
Dog Osteocalcin (OC) ELISA Kit
Product Gene Name
OC elisa kit
Product Synonym Names
BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Species Reactivity
Dog
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.625-40ng/mL
Sensitivity
< 0.249ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of osteocalcin. No significant cross-reactivity or interference between osteocalcin and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level osteocalcin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level osteocalcin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
10,963 Da
OMIM
X53698 mRNA
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Other Notes
Small volumes of OC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for OC purchase
MBS2701833 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteocalcin (OC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OC. The ELISA analytical biochemical technique of the MBS2701833 kit is based on OC antibody-OC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for OC. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
osteocalcin preproprotein
NCBI Official Synonym Full Names
bone gamma-carboxyglutamate protein
NCBI Official Symbol
BGLAP
NCBI Official Synonym Symbols
OC; BGP; OCN
UniProt Protein Name
Osteocalcin
UniProt Synonym Protein Names
Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
NCBI Protein Information
osteocalcin
NCBI Summary for OC
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Show more UniProt Comments for OC
osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.
Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular space; Golgi lumen; perikaryon; rough endoplasmic reticulum; vesicle
Molecular Function: calcium ion binding; hydroxyapatite binding; structural constituent of bone; structural molecule activity
Biological Process: bone mineralization; cell adhesion; cell aging; ER to Golgi vesicle-mediated transport; odontogenesis; osteoblast development; osteoblast differentiation; peptidyl-glutamic acid carboxylation; regulation of bone mineralization; regulation of bone resorption; regulation of osteoclast differentiation; response to activity; response to drug; response to estrogen; response to ethanol; response to glucocorticoid stimulus; response to gravity; response to hydroxyisoflavone; response to mechanical stimulus; response to testosterone stimulus; response to vitamin D; response to vitamin K; response to zinc ion; signal peptide processing; skeletal system development
Show more Protein type: Cell development/differentiation; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: cytoplasm; dendrite; endoplasmic reticulum lumen; extracellular space; Golgi lumen; perikaryon; rough endoplasmic reticulum; vesicle
Molecular Function: calcium ion binding; hydroxyapatite binding; structural constituent of bone; structural molecule activity
Biological Process: bone mineralization; cell adhesion; cell aging; ER to Golgi vesicle-mediated transport; odontogenesis; osteoblast development; osteoblast differentiation; peptidyl-glutamic acid carboxylation; regulation of bone mineralization; regulation of bone resorption; regulation of osteoclast differentiation; response to activity; response to drug; response to estrogen; response to ethanol; response to glucocorticoid stimulus; response to gravity; response to hydroxyisoflavone; response to mechanical stimulus; response to testosterone stimulus; response to vitamin D; response to vitamin K; response to zinc ion; signal peptide processing; skeletal system development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with OC elisa kit
| Reference Product | PubMed Publications |
|---|---|
| RUNX2 elisa kit | >27 publications with OC and RUNX2 |
| BMP2 elisa kit | >9 publications with OC and BMP2 |
| TGFB1 elisa kit | >3 publications with OC and TGFB1 |
| VDR elisa kit | >3 publications with OC and VDR |
| LEP elisa kit | >1 publications with OC and LEP |
| PTHLH elisa kit | >1 publications with OC and PTHLH |
| PTH elisa kit | >1 publications with OC and PTH |
| ATF4 elisa kit | >1 publications with OC and ATF4 |
Pathways associated with OC elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Cell Cycle Pathway antibodies | Cell Cycle Pathway Diagram |
| Cell Cycle, Mitotic Pathway antibodies | Cell Cycle, Mitotic Pathway Diagram |
| FGF Signaling Pathway antibodies | FGF Signaling Pathway Diagram |
| Glucocorticoid Receptor Regulatory Network Pathway antibodies | Glucocorticoid Receptor Regulatory Network Pathway Diagram |
| Interleukin-11 Signaling Pathway antibodies | Interleukin-11 Signaling Pathway Diagram |
| M Phase Pathway antibodies | M Phase Pathway Diagram |
| Mitotic Anaphase Pathway antibodies | Mitotic Anaphase Pathway Diagram |
| Mitotic Metaphase And Anaphase Pathway antibodies | Mitotic Metaphase And Anaphase Pathway Diagram |
| Mitotic Prometaphase Pathway antibodies | Mitotic Prometaphase Pathway Diagram |
| Notch-mediated HES/HEY Network Pathway antibodies | Notch-mediated HES/HEY Network Pathway Diagram |
Diseases associated with OC elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Bone Diseases Antibodies | >8 publications with OC and Bone Diseases |
| Osteoporosis Antibodies | >5 publications with OC and Osteoporosis |
| Neoplasms Antibodies | >4 publications with OC and Neoplasms |
| Hemorrhage Antibodies | >1 publications with OC and Hemorrhage |
| Pain Antibodies | >1 publications with OC and Pain |
| Hypertension Antibodies | >1 publications with OC and Hypertension |
| Cardiovascular Diseases Antibodies | >1 publications with OC and Cardiovascular Diseases |
| Inflammation Antibodies | >1 publications with OC and Inflammation |
| Necrosis Antibodies | >1 publications with OC and Necrosis |
| Brain Injuries Antibodies | >1 publications with OC and Brain Injuries |
Organs/Tissues associated with OC elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Bone Antibodies | >49 publications with OC and Bone |
| Brain Antibodies | >3 publications with OC and Brain |
| Parathyroid Antibodies | >1 publications with OC and Parathyroid |
| Prostate Antibodies | >1 publications with OC and Prostate |
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