Dolichos biflorus (DBA, Horse gram) (FITC)

Référence D8085-15-1mg

Conditionnement : 1mg

Marque : US Biological

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D8085-15 Dolichos biflorus (DBA, Horse gram) (FITC)

Grade
Molecular Biology Grade
Applications
IHC
Shipping Temp
Blue Ice
Storage Temp
-20°C

Lectins are derived from the extracts of plants, animals, viruses and microorganisms. They are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Carbohydrate Specificity: Methyl-2-acetamido-2-deoxy-D-galactose.

Inhibitory Carbohydrate: Terminal alpha-D-Acetylfalactosamine.

Acitivity:
4ug/ml will agglutinate human type A1 cells. ≥200ug/ml is needed to agglutinate human type A2 cells.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Source: Horse Gram|Purity: Gel filtration performed after conjugation to remove free FITC. Quality Control: SDS-PAGE|Concentration: ~1mg/ml |Form: Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide. Labeled with FITC. FITC:Protein Ratio: 1.0-2.0||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide. Labeled with FITC. FITC:Protein Ratio: 1.0-2.0
Purity
Gel filtration performed after conjugation to remove free FITC. Quality Control: SDS-PAGE
References
1. Bird G.W. Current Science 20:298 (1951) 2. Etzler M.E. et al., Biochemistry 9: 869-877 (1970) 3. Hammerstrom et al., Biochchemistry (1977)