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> Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder)

Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder)

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Référence MBS652825-500g

Conditionnement : 500g

Marque : MyBiosource

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Drop-out Mix Complete, Adenine Low Culture Media

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS652825

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. More details

Product Overview

Online Datasheet in PDF
Product Name
Drop-out Mix Complete, Adenine Low, Culture Media
Full Product Name
Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder)
Form/Format
White, homogeneous, free flowing powder
Purity/Purification
Molecular Biology Grade
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Solubility
Colorless, clear, complete
pH
4.15
Directions per Liter
Mix 2 grams of drop-out mix with 26.7g drop-out base with carbohydrate (YNB) (or 6.7g drop-out base with out carbohydrate) (or 43.7g drop-out base with agar) per liter of distilled/ deionized water. Heat with stirring until completely solubilized. Adjust to desired pH. Sterilize with 0.2um membrane filter. Aliquot into sterile containers . Do not autoclave, contains heat-liable compounds that can be damaged with aoutclaving.
Media Formulation
Each bottle is a homogeneous mixture of the following amino acids and nucleotide bases.
Amino Acids
Alanine 2.0g
p-Aminobenzoic acid 0.2g
Arginine 2.0g
Asparagine 2.0g
Aspartic acid 2.0g
Cysteine 2.0g
Glutamic acid 2.0g
Glutamine 2.0g
Glycine 2.0g
Histidine 2.0g
Isoleucine 2.0g
Leucine 10.0g
Lysine 2.0g
Methionine 2.0g
Phenylalanine 2.0g
Proline 2.0g
Serine 2.0g
Threonine 2.0g
Tryptophan 2.0g
Tyrosine 2.0g
Valine 2.0g

Nucleotide Bases:
*Adenine 0.118g
Inositol 2.0g
Uracil 2.0g

Use 2g/liter
100g makes ~50 liters

*Final concetration of Adenine is 4.5mg/liter

Product Categories/Family for Drop-out Mix Complete, Adenine Low culture media

Culture Media; Media-Yeast

Other Notes

Small volumes of Drop-out Mix Complete, Adenine Low culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Drop-out Mix Complete, Adenine Low purchase

MBS652825 is a culture media targeting Drop-out Mix Complete, Adenine Low. Also known as Drop-out Mix Complete, Adenine Low (0.118g) w/o Yeast Nitrogen Base (Powder).