Dylight 594, Goat Anti-Rabbit IgG
Référence A23420-100
Conditionnement : 100uL
Marque : Abbkine
Specification
Product name | Dylight 594, Goat Anti-Rabbit IgG |
Immunogen | Rabbit IgG whole molecule |
Host | Goat |
Reactivity | Rabbit |
Applications | FCM, ICC, IF |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Preparation method | Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE |
Conjugate | Dylight 594 |
Product Properties
Formulation | Liquid solution |
Features & Benefits | DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9). |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
Background | Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples. |
Others | The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species. |
Image & description
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Increased expression of Sonic hedgehog restores diabetic endothelial progenitor cells and improves cardiac repair after acute myocardial infarction in diabetic mice(A23420-2.91)
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Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis(A23420-2.9)
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MicroRNA-155 Suppresses Mesangial Cell Proliferation and TGF-β1 Production via Inhibiting CXCR5-ERK Signaling Pathway in Lupus Nephritis(A23420-2.84)
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Glioma cells escaped from cytotoxicity of temozolomide and vincristine by communicating with human astrocytes(A23420-2.83)
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Cytochrome c1-like is required for mitochondrial morphogenesis and individualization during spermatogenesis in Drosophila melanogaster(A23420-2.8)
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Moderate oxidative stress promotes epithelial–mesenchymal transition in the lens epithelial cells via the TGF-β/Smad and Wnt/β-catenin pathways(A23420-2.795)
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Small RNA analysis provides new insights into cytoplasmic incompatibility in Drosophila melanogaster induced by Wolbachia(A23420-2.75)
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Activation of EphA1-Epha receptor axis attenuates diabetic nephropathy in mice(A23420-2.69)
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Low expression of IL-37 protein is correlated with high Oct4 protein expression in hepatocellular carcinoma(A23420-2.6)
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Protein Kinase C γ Contributes to Central Sensitization in a Rat Model of Chronic Migraine(A23420-2.57)
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Establishment and characterization of a carcinoma-associated fibroblast cell line derived from a human salivary gland adenoid cystic carcinoma(A23420-2.50)
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Resveratrol transcriptionally regulates miRNA-18a-5p expression ameliorating diabetic nephropathy via increasing autophagy(A23420-2.21)
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Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis via NF-κB signaling pathway(A23420-2.172)
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G-CSF reduces loss of dopaminergic neurons by inhibiting TNF-α and IL-1β in mouse model of Parkinson's disease(A23420-2.107)
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Upregulation of antioxidant and autophagy pathways via NRF2 activation protects spinal cord neurons from ozone damage(A23420-2.1)
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Melatonin inhibits proliferation and viability and promotes apoptosis in colorectal cancer cells via upregulation of the microRNA-34a/449a cluster(A23420-2.1)
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Knockdown of Herp alleviates hyperhomocysteinemia mediated atherosclerosis through the inhibition of vascular smooth muscle cell phenotype switching(A23420-2.01)
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Partial deficiency of HIF-1α in chondrocytes effected bone repair of mandibular condylar neck(A23420-1.931)
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Decreased IL‑37 expression in hepatocellular carcinoma tissues and liver cancer cell lines(A23420-1.84)
Author:P Li, H Guo, K Wu Publication name:Oncology Letters IF:1.84
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Activation of A4B adenosine receptor protects against demyelination in a mouse model of schizophrenia(A23420-1.785)
Author:Ma Q, Wang D, Li Y Publication name:Exp Ther Med IF:1.785
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Inhibition of cGAS aggravated the host inflammatory response to Aspergillus fumigatus(A23420-1.7)
Author:Peng, Mei, et al. Publication name:Experimental Lung Research IF:1.7
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miR-145-5p suppresses osteogenic differentiation of adipose-derived stem cells by targeting semaphorin 3A(A23420-1.447)
Author:Xiangdong Liu, Wenzhong Zhu, Lei Wang, et al Publication name:In Vitro Cell Dev Biol Anim IF:1.447
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Berberine Reshapes the Balance of the Local Renin-Angiotensin System by Modulating Autophagy under Metabolic Stress in Pancreatic Islets(A23420-1.417)
Author:Huang C, Lei P, Peng C, Li M, Guo Y, Li X Publication name:J Renin Angiotensin Aldosterone Syst IF:1.417
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miR-103a-3p Suppresses Cell Proliferation and Invasion by Targeting Tumor Protein D52 in Prostate Cancer(A23420-1.07)
Author:J Ge, L Mao, W Xu Publication name:Journal of Investigative Surgery IF:1.07
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The Ultrastructure of MCF-7 Breast Cancer Cells after Vasodilator-Stimulated Phosphoprotein Knockdown(A23420-0.96)
Author:Wang Y, Su K, Hu P C, et al Publication name:Ultrastructural pathology IF:0.96
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Downregulation of inwardly rectifying potassium channel 5.1 expression in C57BL/6J cochlear lateral wall(A23420-)
Author:Pan C, Chu H, Lai Y, et al Publication name:Journal of Huazhong University of Science and Technology [Medical Sciences] IF:
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Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation(A23420-)
Author:Wei Zhong, Bo Li, et al Publication name:JACC Basic to Translational Science IF:
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Decreased miR-29b expression is associated with airway inflammation in chronic obstructive pulmonary disease(A23420-)
Author:Kun Tang, Jianping Zhao, Jungang Xie, Jianmiao Wang Publication name:Am J Physiol Lung Cell Mol Physiol IF:
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MBD2 facilitates tumor metastasis by mitigating DDB2 expression(A23420-/)
Author:Zhang L, Wang S, Wu G R, et al. Publication name:Research Square IF:/
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