Dynein, Cytoplasmic, 74kD Intermediate Chains (HRP)

Applications:
Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Immunocytochemistry: Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells.
Western Blot: Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells.
Immunoprecipitation: immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.