EGFR Protein, Human, Recombinant (His)

1.Measured by its binding ability in a functional ELISA. Immobilized Human EGF hFc at 2 μg/ml (100 μl/well) can bind Human EGFR His , the EC50 of Human EGFR His is 100-500 ng/mL.
2. Captured Cetuximab (IgG1) on proA Chip can bind Human EGFR recombinant protein with an affinity constant of 1.07 nM as determined in an SPR assay (Routinely tested).
3. Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His Tag with an affinity constant of 5.81nM as determined in BLI assay (Routinely tested).

EGFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.8 kDa and the accession number is P00533-1.

Human

HEK293 Cells

> 95 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.