EnzyFluo™ Fatty Acyl-CoA Assay Kit
Application
- For quantitative determination of fatty acyl-CoAs in tissue and cell lysates.
Key Features
- Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.3 to 100 µM fatty acyl-CoA. The internal standard method minimizes potential sample matrix interferences.
- Fast and convenient. Room temperature assay with a 40 min incubation. No 37°C incubator is needed.
- High-throughput. Homogeneous “mix-incubate-measure” type assay. It can be readily automated to assay thousands of samples per day.
Method
- FL530/585nm
Samples
- Tissue and cell lysates
Species
- All
Procedures
- 40 min
Size
- 100 tests in 96-well plate
Detection Limit
- 0.3 µM
Shelf Life
- 6 months
More Details
Fatty Acyl-CoAs are a combination of Coenzyme A covalently bound to long-chain fatty acids. Fatty acyl-CoAs serve as both a precursor for triglyceride and phospholipids synthesis and a product of lipid catabolism. Catabolically, they facilitate the transport of fatty acids to the mitochondria for shuttling via the carnitine shuttle and subsequent β-oxidation of the fatty acid. Conversion of fatty acids to fatty acyl-CoAs is necessary for the generation of ATP during periods of high energy demand. Elevated fatty acyl-CoA levels may indicate dysregulation of lipid metabolism such as due to insulin resistance. Certain genetic disorders may also affect fatty acyl-CoA levels. BioAssay Systems’ fluorimetric fatty acyl-CoA assay works using a combination of enzymes that utilize fatty acyl-CoA as a substrate, which is coupled to form a fluorescent product. The resulting fluorescence intensity at λexc/em = 530/585 nm is linear to the fatty acyl-CoA concentration in the sample.How should I store my samples?
Fatty acyl-CoAs are not stable and samples should ideally be tested the same day. Otherwise, snap freeze the samples in liquid nitrogen or freeze at -80°C for up to one week.
Can this kit be used with serum or plasma samples?
In our testing, the concentration of fatty acyl-CoAs was too low to detect in these samples.
Can I use a different lysis buffer?
We suggest using a lysis buffer that is pH 7.4 and is 0.5% – 5.0% triton X-100 buffer to dissolve fatty acyl-CoAs.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
