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Product Description
This antibody is a mixture of four different monoclonal antibodies. This antibody is specific to 60 kDa latent membrane protein (LMP-1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic C-terminus of the cytoplasmic domain of LMP-1. This antibody stains strongly with EBV-positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis. EBV, also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV infects B cells and, though often asymptomatic, it can cause infectious mononucleosis, a disease characterized by fatigue, fever, sore throat and muscle soreness.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF ® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.
Catalog number key for antibody number 1742, Anti-Epstein-Barr Virus (CS2)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified,
BSA-free | N/A | N/A | N/A | |
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.
Technology
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Product Attributes
Antibody reactivity (target)
Epstein-Barr Virus
Synonyms
EBNA1; EBV nuclear antigen 1; Epstein Barr nuclear antigen 1; Human Herpesvirus 4 (HHV4)
Human gene symbol
Not Applicable
Entrez gene ID
Not Applicable
Immunogen
Recombinant fusion protein containing the sequence of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP
Antibody target cellular localization
Nucleus
Expected antibody applications
IHC (frozen) (published for clone), IHC (FFPE) (published for clone), IP (published for clone), WB (published for clone)
Antibody application notes
ELISA: For coating, purchase antibody without BSA; Western blot 1-2 ug/mL; Optimal dilution for a specific application should be determined., Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
Positive control
EBV-infected cells. Tissues.
Shipping condition
Room temperature
Storage Conditions
Note: store BSA-free antibodies at -10 to -35°C, Store at 2 to 8 °C, Protect fluorescent conjugates from light
Regulatory status
For research use only (RUO)
Antibody/conjugate formulation
Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Shelf life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Product origin
Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Documents, Protocols, SDS and COA
Datasheet
Datasheet Download
COARequest the Certificate of Analysis
References
Note: References for this clone sold by other suppliers may be listed for expected applications. - J Exp Med (1993) 177: 339-349. (IHC, frozen; WB; mix of clones CS1-4)
- J Clin Pathol (1995) 48:438-442. (IHC, FFPE; mix of clones CS1-4)
- EMBO J (1999)18:3064-3073. (IP; mix of clones CS1-4)
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