Epstein-Barr Virus (VCA) IgM ELISA

Principle of the Assay: The immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiterplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA Microtiterplate reader.

Intended Uses: The Epstein-Barr Virus (VCA) IgM ELISA is intended for the determination of IgM class antibodies against Epstein-Barr Virus viral capsid antigen (VCA) in human serum or plasma (citrate, heparin). For research use only - Not for use in test procedures

Small volumes of VCA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495649 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epstein-Barr Virus (VCA) IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VCA. The ELISA analytical biochemical technique of the MBS495649 kit is based on VCA antibody-VCA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).