Quantibody® Equine Cytokine Array 1 Kit. Detects 10 Equine Cytokines. Suitable for all liquid sample types.
Equine Cytokine Array Q1
Equine Cytokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Equine (horse) |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 Ra (IL-1 F3)
| IL-2
| IL-4
|
IL-8 (CXCL8)
| IL-10
| IL-15
| MCP-1 (CCL2)
| VEGF-A
|
Application Notes
Suggested Application
- Multiplexed Protein Detection
- Biomarker Screening
- Identifying Key Factors
- Confirming Biological Process
- Biomarker Validation
- Validation of Antibody Array Results
- Quantitative Protein Detection
- Establishing Normal Range
Kit Components
- Quantibody® Bovine Cytokine Array 1 Slide
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAE-CYT-1.gal)
Excel (QAE-CYT-1_Analysis_Tool.xls)
citations
Exogenous interleukin-1 beta stimulation regulates equine tenocyte function and gene expression in three-dimensional culture which can be rescued by pharmacological inhibition of interleukin 1 receptor, but not nuclear factor kappa B, signaling
- Species:
- Equine
- Sample Type:
- tenocytes
- Year:
- 2023
- Journal Name:
- Molecular and Cellular Biochemistry
Effects of intra-articular injection of an acellular equine liquid amniotic allograft in healthy equine joints
- Species:
- Horse
- Sample Type:
- Synovial fluid
- Year:
- 2022
- Journal Name:
- Veterinary Surgery