ExoBrite™ 560/585 IgG1 Isotype Control Flow Antibody (25 tests)
Référence P008-560-125
Conditionnement : 125UL
Marque : Biotium
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Téléphone : +1 850 650 7790
ExoBrite™ IgG1 Isotype Control Flow Antibody
Non-reactive mouse antibody that serves as a negative control for mouse ExoBrite™ Flow Antibodies when detecting purified or bead-bound EVs by flow cytometry.
Product Description
ExoBrite™ IgG1 Isotype Control Flow Antibody has been found to not react with any target in human cells and serves as a negative control for mouse ExoBrite™ Flow Antibodies. The antibody is available with the same conjugates as ExoBrite™ Flow Antibodies for the Pacific Blue™, FITC, PE, and APC channels.
- Non-reactive IgG1 mouse antibody
- Serves as a isotype negative control for ExoBrite™ Flow Antibodies
- Available in 6 colors
Biotium also offers validated ExoBrite™ Flow Antibodies for tetraspanin proteins CD9, CD63, CD81 and other EV markers.
EV antibodies you can trust
Other commercially available antibodies against mouse and human tetraspanin proteins CD9, CD63, and CD81 are generally not validated for isolated EVs and may require tedious optimization for your EV prep and staining protocol. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise. Single-color and 3-color ExoBrite™ CD9/CD63/CD81 Antibody Cocktails for human tetraspanin proteins are also available for high-coverage EV staining or EV phenotyping, respectively.
For general EV staining, Biotium's ExoBrite™ True EV Membrane Stains offer unparalleled coverage of EVs in a sample and address issues of dye aggregation often seen with PKH and other common membrane dyes. Biotium also offers optimized ExoBrite™ EV Surface stains conjugated to cholera toxin B (CTB), wheat germ agglutinin (WGA), and Annexin V. These stains are specially formulated for bright and specific detection of isolated EVs by flow cytometry. These ExoBrite™ EV Surface stains may also be combined with antibody staining, for multi-parameter analysis.
Biotium offers conjugated ExoBrite™ Western Antibodies against human CD9, CD63, and CD81 designed for optimal detection in EV extracts by fluorescent western blot.
Note: In our testing, we have found that ExoBrite™ 490/515 dye may bind to streptavidin coated surfaces or beads if free biotin binding sites are not blocked. We recommend performing a biotin blocking step after binding your biotinylated capture antibody to streptavidin beads or surfaces when using ExoBrite™ 490/515 conjugates. Alternatively, consider using a different ExoBrite™ dye for staining EVs captured on streptavidin beads or surfaces.
ExoBrite™ Flow Antibody Conjugates
ExoBrite™ Flow Anti-Human CD9/CD63/CD81 Antibody Cocktails
| Cocktail | Ex/Em | Detection Channel | Size | Catalog No. |
|---|---|---|---|---|
| ExoBrite™ CD9/CD63/CD81 Single-Color Antibody Cocktail (Green) | 490/516 nm | FITC | 25 tests | P030-125 |
| 100 tests | P030-500 | |||
| ExoBrite™ CD9/CD63/CD81 Single-Color Antibody Cocktail (Orange) | 562/584 nm | PE | 25 tests | P031-125 |
| 100 tests | P031-500 | |||
| ExoBrite™ CD9/CD63/CD81 Single-Color Antibody Cocktail (Far-Red) | 642/663 nm | APC | 25 tests | P032-125 |
| 100 tests | P032-500 | |||
| ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Blue/Green/Far-Red) | 409/452 nm 490/516 nm 642/663 nm | Pacific Blue® FITC APC | 25 tests | P028-125 |
| 100 tests | P028-500 | |||
| ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Green/Orange/Far-Red) | 490/516 nm 562/584 nm 642/663 nm | FITC PE APC | 25 tests | P029-125 |
| 100 tests | P029-500 |
ExoBrite™ Antibody Target Info
| Target | Relevance in EVs | References for Target in EVs | Antibody |
|---|---|---|---|
| CD9 | Tetraspanin; common EV marker | Kowal et al., 2016; Théry et al., 2018; Spitzberg et al., 2023 | ExoBrite™ CD9 Flow Antibody |
| ExoBrite™ CD9 (Mouse) Flow Antibody | |||
| ExoBrite™ CD9 Western Antibody | |||
| CD29 (Integrin beta 1) | Integrin cell surface glycoprotein found on EV surfaces. Has broad tissue expression and commonly found in EV proteomic analysis. | Hoshino et al., 2015; Hazawa et al., 2014; Kugeratski et al., 2021; Spitzberg et al., 2023 | ExoBrite™ CD29 Flow Antibody |
| CD47 | Associates with integrins; regulates trafficking of RNAs into EVs; may contribute to immune evasion on target cells. Has broad tissue expression and commonly found in EV proteomic analysis. | Kamerkar et al., 2017; Li et al., 2022; Kugeratski et al., 2021; Spitzberg et al., 2023 | ExoBrite™ CD47 Flow Antibody |
| CD63 | Tetraspanin; common EV marker | Escola et al., 1998; Kowal et al., 2016; Spitzberg et al., 2023 | ExoBrite™ CD63 Flow Antibody |
| ExoBrite™ CD63 (Mouse) Flow Antibody | |||
| ExoBrite™ CD63 Western Antibody | |||
| CD81 | Tetraspanin; common EV marker | Escola et al., 1998; Kowal et al., 2016; Spitzberg et al., 2023 | ExoBrite™ CD81 Flow Antibody |
| ExoBrite™ CD81 (Mouse/Rat) Flow Antibody | |||
| ExoBrite™ CD81 Western Antibody | |||
| CD98 (SLC3A2) | Has broad tissue expression and commonly found in EV proteomic analysis. | Mathieu et al., 2021; Liao et al., 2024; Tissot et al., 2024; Kugeratski et al., 2021; Spitzberg et al., 2023 | ExoBrite™ CD98 Flow Antibody |
| Syntenin-1 | Involved in EV biogenesis, plays a role in tumor growth, invasion, and metastasis | Mathieu et al., 2021; Kugeratski et al., 2021; Spitzberg et al., 2023; Baietti et al., 2012 | ExoBrite™ Syntenin-1 Flow Antibody |
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