Fast Red Super
Référence MBS258526-20
Conditionnement : 20Tablets
Marque : MyBiosource
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Fast Red Super Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258526Our unique Fast Red Super is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC) with alkaline phosphatase. This chromogen system gives red color and is very compatible with hematoxylin counter stains. This… More details
Product Overview
Online Datasheet in PDFProduct Name
Fast Red Super, Reagent
Full Product Name
Fast Red Super
Preparation and Storage
Store at 2-8 degree C
Kit Contents
Reagent B: buffer, 10 ml
Reagent C: chromogen, 20 tablets
Reagent S; substrate, 5 ml
Reagent C: chromogen, 20 tablets
Reagent S; substrate, 5 ml
Product Categories/Family for Fast Red Super reagent
IHC Reagents
Applications Tested/Suitable for Fast Red Super reagent
Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for Fast Red Super reagent
Mixing of Reagent:
1. To 4.9 ml of distilled or deionized water in a test tube, add five drops of buffer reagent B, mix well.
2. Add Fast Red tablet (Reagent C), let stand in this solution for 5-10 minutes. Vortex for 3-5 minutes till the entire tablet is dissolved.
3. Add 5 drops of substrate reagent S, mix. (This ready to use reagent is good for several hours. The unused solution can be discarded according to city, county, state, province or country's regulations.
Application: IHC, ICC
1. After alkaline phosphatase reagent, wash slide with buffer and distilled/deionized water.
2. Add few drops of ready to use Fast Red reagent; incubate at RT or 37 degree C for 5-10 minutes. The optimum time of incubation for Fast red Super should be determined by the individual lab.
3. Wash slides 2-5X with buffer, followed by rinsing with distilled/deionized water.
4. Add counterstain compatible with red color. We use hematoxylin for 30-60 seconds, followed by washing with tap water, buffer with pH over 7 to convert purple blue color to blue color.
5. The slide can be mounted with aqueous mounting medium See #6 or organic mounting medium see #7).
6. Wash the slide with distilled/deionized water. The slide can be mounted with aqueous mounting medium (we use ImmunoHistoMount; see data sheet)
7. Or the slide can be mounted with organic mounting medium. (Dehydrate with increasing % of alcohol, 100% alcohol, followed by limonene, toluene, xylene or any other dehydrating reagent. (We use Organo/limonene mounting medium, please follow data sheet for mounting medium).
1. To 4.9 ml of distilled or deionized water in a test tube, add five drops of buffer reagent B, mix well.
2. Add Fast Red tablet (Reagent C), let stand in this solution for 5-10 minutes. Vortex for 3-5 minutes till the entire tablet is dissolved.
3. Add 5 drops of substrate reagent S, mix. (This ready to use reagent is good for several hours. The unused solution can be discarded according to city, county, state, province or country's regulations.
Application: IHC, ICC
1. After alkaline phosphatase reagent, wash slide with buffer and distilled/deionized water.
2. Add few drops of ready to use Fast Red reagent; incubate at RT or 37 degree C for 5-10 minutes. The optimum time of incubation for Fast red Super should be determined by the individual lab.
3. Wash slides 2-5X with buffer, followed by rinsing with distilled/deionized water.
4. Add counterstain compatible with red color. We use hematoxylin for 30-60 seconds, followed by washing with tap water, buffer with pH over 7 to convert purple blue color to blue color.
5. The slide can be mounted with aqueous mounting medium See #6 or organic mounting medium see #7).
6. Wash the slide with distilled/deionized water. The slide can be mounted with aqueous mounting medium (we use ImmunoHistoMount; see data sheet)
7. Or the slide can be mounted with organic mounting medium. (Dehydrate with increasing % of alcohol, 100% alcohol, followed by limonene, toluene, xylene or any other dehydrating reagent. (We use Organo/limonene mounting medium, please follow data sheet for mounting medium).
Other Notes
Small volumes of Fast Red Super reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Fast Red Super purchase
MBS258526 is a reagent targeting Fast Red Super.