OKWB00296 | FETUIN A ELISA Kit (Monkey) Biovalley

Technical Documents

Datasheets/Manuals	Printable datasheet for FETUIN A ELISA Kit (Monkey) (OKWB00296)

Product Info

Predicted Species Reactivity

Chlorocebus aethiops|Monkey

Application

ELISA-Sandwich

ELISA Kit Detection Method

Colorimetric, OD 450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Linearity

Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
Serum (n=5)	87-103%	86-104%	93-104%	89-103%
EDTA Plasma (n=5)	85-96%	89-96%	85-100%	82-95%
Heparin Plasma (n=5)	84-93%	80-89%	82-95%	84-98%

ELISA Kit Principle

Aviva Systems Biology Fetuin a ELISA Kit (Monkey) (OKWB00296) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fetuin a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fetuin a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fetuin a captured in the well.

ELISA Kit Range

6.25-400ng/ml

ELISA Kit Recovery

Sample Type	Range	Mean
Serum (n=5)	89-105	96
EDTA Plasma (n=5)	90-104	98
Heparin Plasma (n=5)	87-101	93

ELISA Kit Reproducibility

Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%

ELISA Kit Component

Component	Amount
Fetuin a Microplate	96 Wells (12 x 8 Well strips)
Fetuin a Lyophilized Standard	2 x 400 ng
100X Fetuin a Biotinylated Detector Anitbody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Standard Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids

Sensitivity

3.75 ng/mL

Assay Info

Assay Type: Quantitative Sandwich ELISA

Target Info

Gene Symbol	FETUIN A
Protein Name	Fetuin-A
Uniprot ID	B9MSS1

Protocols & Data

FETUIN A ELISA Kit (Monkey)

Référence OKWB00296

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FETUIN A ELISA Kit (Monkey) (OKWB00296)