USE: Add one DISC into one well of a 6-well plate, change DISC once a week and feed with your regular medium 2-3 times a week.
Note: Standard size DISCs release at 10 ng/mL in 2 mL of medium; Mini size DISCs release at 10 ng/mL in 1 mL of medium. See table below to determine which DISC size suits your needs.