FITC-Linked Polyclonal Antibody to Vinculin (VCL)

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: adherens junction; cell-cell adherens junction; costamere; cytoskeleton; cytosol; extracellular region; focal adhesion; intercellular junction; protein complex

Molecular Function: actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding; ubiquitin protein ligase binding

Biological Process: apical junction assembly; cell adhesion; cell motility; cell-matrix adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; muscle contraction; negative regulation of cell migration; platelet degranulation

Disease: Cardiomyopathy, Dilated, 1w