FOS siRNA (Mouse)
Référence MBS829666-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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FOS siRNA – Proto-Oncogene C-Fos (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS829666MBS829666 is an siRNA targeting FOS. Also known as FOS siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit FOS expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
FOS, siRNA
Full Product Name
FOS siRNA (Mouse)
Product Gene Name
FOS sirna
Product Synonym Names
Proto-oncogene c-Fos; Cellular oncogene fos
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FOS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
40,838 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse FOS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FOS sirna
RNA Interference (RNAi)
Other Notes
Small volumes of FOS sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for FOS. It may not necessarily be applicable to this product.
NCBI Official Full Name
proto-oncogene c-Fos
NCBI Official Synonym Full Names
FBJ osteosarcoma oncogene
NCBI Official Symbol
Fos
NCBI Official Synonym Symbols
cFos; c-fos; D12Rfj1
UniProt Protein Name
Proto-oncogene c-Fos
UniProt Synonym Protein Names
Cellular oncogene fos
Protein Family
Proto-oncogene
UniProt Gene Name
Fos
UniProt Entry Name
FOS_MOUSE
NCBI Protein Information
proto-oncogene c-Fos
UniProt Comments for FOS
Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.
Protein type: Oncoprotein; DNA-binding; Transcription factor; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; response to drug; nervous system development; regulation of transcription, DNA-dependent; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; cellular response to extracellular stimulus; positive regulation of transcription from RNA polymerase II promoter
Show more Protein type: Oncoprotein; DNA-binding; Transcription factor; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm; transcription factor complex; neuron projection; membrane; endoplasmic reticulum; cytoplasm; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; chromatin binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; response to drug; nervous system development; regulation of transcription, DNA-dependent; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; cellular response to extracellular stimulus; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with FOS sirna
| Reference Product | PubMed Publications |
|---|---|
| Jun sirna | >8219 publications with FOS and Jun |
| Junb sirna | >716 publications with FOS and Junb |
| Egr1 sirna | >628 publications with FOS and Egr1 |
| Tnf sirna | >581 publications with FOS and Tnf |
| Jund sirna | >503 publications with FOS and Jund |
| Creb1 sirna | >59 publications with FOS and Creb1 |
| Mapk8 sirna | >46 publications with FOS and Mapk8 |
| Mapk14 sirna | >9 publications with FOS and Mapk14 |
| Mapk9 sirna | >1 publications with FOS and Mapk9 |
Pathways associated with FOS sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Activated TLR4 Signalling Pathway antibodies | Activated TLR4 Signalling Pathway Diagram |
| Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies | Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram |
| Amphetamine Addiction Pathway antibodies | Amphetamine Addiction Pathway Diagram |
| Amphetamine Addiction Pathway antibodies | Amphetamine Addiction Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| B Cell Receptor Signaling Pathway antibodies | B Cell Receptor Signaling Pathway Diagram |
| Cellular Senescence Pathway antibodies | Cellular Senescence Pathway Diagram |
| Cellular Responses To Stress Pathway antibodies | Cellular Responses To Stress Pathway Diagram |
| Chagas Disease (American Trypanosomiasis) Pathway antibodies | Chagas Disease (American Trypanosomiasis) Pathway Diagram |
| Chagas Disease (American Trypanosomiasis) Pathway antibodies | Chagas Disease (American Trypanosomiasis) Pathway Diagram |
Diseases associated with FOS sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >2889 publications with FOS and Nervous System Diseases |
| Pain Antibodies | >1466 publications with FOS and Pain |
| Inflammation Antibodies | >1038 publications with FOS and Inflammation |
| Necrosis Antibodies | >762 publications with FOS and Necrosis |
| Epilepsy Antibodies | >530 publications with FOS and Epilepsy |
| Seizures Antibodies | >513 publications with FOS and Seizures |
| Hyperalgesia Antibodies | >410 publications with FOS and Hyperalgesia |
| Hypertension Antibodies | >381 publications with FOS and Hypertension |
| Liver Diseases Antibodies | >380 publications with FOS and Liver Diseases |
| Breast Neoplasms Antibodies | >372 publications with FOS and Breast Neoplasms |
Organs/Tissues associated with FOS sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >11227 publications with FOS and Brain |
| Muscle Antibodies | >1490 publications with FOS and Muscle |
| Spinal Cord Antibodies | >1473 publications with FOS and Spinal Cord |
| Bone Antibodies | >1288 publications with FOS and Bone |
| Liver Antibodies | >1005 publications with FOS and Liver |
| Skin Antibodies | >696 publications with FOS and Skin |
| Lung Antibodies | >592 publications with FOS and Lung |
| Eye Antibodies | >587 publications with FOS and Eye |
| Intestine Antibodies | >546 publications with FOS and Intestine |
| Bone Marrow Antibodies | >444 publications with FOS and Bone Marrow |